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Tobacco Agar, a New Medium for Differentiating Candida dubliniensis from Candida albicans

Department of Microbiology, Faculty of Medicine, Kuwait University, Safat 13110, Kuwait

Received 19 January 2004/ Returned for modification 25 February 2004/ Accepted 2 June 2004

Isolates of Candida dubliniensis may be misidentified as Candida albicans in microbiological laboratories if only the germ tube and/or the chlamydospore test is used for identification to the species level. In this study, we have evaluated the efficacy of tobacco agar for the differentiation of C. dubliniensis from C. albicans. On this medium at 28°C, all 30 C. dubliniensis isolates produced yellowish-brown colonies with hyphal fringes and abundant chlamydospores, whereas 54 C. albicans isolates formed smooth, white-to-cream-colored colonies with no chlamydospore production. This medium provides a simple tool for presumptive differentiation of C. dubliniensis from C. albicans.

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