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Journal of Clinical Microbiology, October 2004, p. 4812-4814, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4812-4814.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of an Immunochromatography Test with Multiplex Reverse Transcription-PCR for Rapid Diagnosis of Respiratory Syncytial Virus Infections

Yuki Kuroiwa,1* Kazushige Nagai,1 Lisa Okita,1 Susumu Ukae,2 Toshihiko Mori,3 Tomoyuki Hotsubo,4 and Hiroyuki Tsutsumi1

Department of Pediatrics, Sapporo Medical University School of Medicine,1 Department of Pediatrics, Sapporo Social Insurance General Hospital,2 Department of Pediatrics, NTT East Sapporo Hospital,3 Department of Pediatrics, Tonan Hospital, Sapporo, Japan4

Received 22 May 2004/ Returned for modification 1 July 2004/ Accepted 20 July 2004

A new commercial rapid 10-min one-step immunochromatography (IC) test, SAS RSV test, was compared to another IC test, Directigen EZ RSV, employing RT-PCR as the "gold standard" for detecting respiratory syncytial virus. Of 102 clinical samples, 79 were positive by RT-PCR, 66 (82.5%) were positive with the SAS RSV test, and 55 (69.6%) were positive with Directigen EZ RSV. The specificity of the new test was 91.3% (21 of 23), similar to that of Directigen EZ RSV (100% [23 of 23]). This test performs well enough to be used for patient care.

* Corresponding author. Mailing address: Department of Pediatrics, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, Hokkaido 060-8543, Japan. Phone: 81-11-611-2111. Fax: 81-11-611-0352. E-mail: yukik{at}mb.infosnow.ne.jp.

Journal of Clinical Microbiology, October 2004, p. 4812-4814, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4812-4814.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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