This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mellmann, A. Articles by Harmsen, D. Search for Related Content PubMed PubMed Citation Articles by Mellmann, A. Articles by Harmsen, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2004, p. 4840-4842, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4840-4842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations

Institut für Hygiene, Universitätsklinikum Münster, Münster,¹ Institut für Hygiene und Umwelt, Hamburg,³ Bundesinstitut für Risikobewertung,² Robert Koch-Institut, Berlin, Germany⁴

Received 17 March 2004/ Returned for modification 20 May 2004/ Accepted 20 June 2004

Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

This article has been cited by other articles:

• Price, E. P., Huygens, F., Giffard, P. M. (2006). Fingerprinting of Campylobacter jejuni by Using Resolution-Optimized Binary Gene Targets Derived from Comparative Genome Hybridization Studies. Appl. Environ. Microbiol. 72: 7793-7803 [Abstract] [Full Text]  
• Price, E. P., Thiruvenkataswamy, V., Mickan, L., Unicomb, L., Rios, R. E., Huygens, F., Giffard, P. M. (2006). Genotyping of Campylobacter jejuni using seven single-nucleotide polymorphisms in combination with flaA short variable region sequencing.. J Med Microbiol 55: 1061-1070 [Abstract] [Full Text]