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Journal of Clinical Microbiology, October 2004, p. 4840-4842, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4840-4842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequence-Based Typing of flaB Is a More Stable Screening Tool Than Typing of flaA for Monitoring of Campylobacter Populations

Alexander Mellmann,1 Jan Mosters,1 Edda Bartelt,2 Peter Roggentin,3 Andrea Ammon,4 Alexander W. Friedrich,1 Helge Karch,1 and Dag Harmsen1*

Institut für Hygiene, Universitätsklinikum Münster, Münster,1 Institut für Hygiene und Umwelt, Hamburg,3 Bundesinstitut für Risikobewertung,2 Robert Koch-Institut, Berlin, Germany4

Received 17 March 2004/ Returned for modification 20 May 2004/ Accepted 20 June 2004

Different typing schemes for Campylobacter spp. were evaluated with 70 outbreak and sporadic isolates. The discriminatory indexes were 0.944 (by pulsed-field gel electrophoresis), 0.920 (by genotyping of the flagellin A gene), 0.902 (by genotyping of flaB), and 0.886 (by multilocus sequence typing). Cross-classification gave 94.77 or 95.82% (PFGE-flaA or PFGE-flaB) concordance. flaA was overdiscriminatory in three cases, most probably due to intragenomic recombination.

* Corresponding author. Mailing address: Institut für Hygiene, Universitätsklinikum Münster, Robert Koch-Str. 41, D-48149 Münster, Germany. Phone: 49-251-83-5-53-48. Fax: 49-251-83-5-56-88. E-mail: dharmsen{at}uni-muenster.de.

Journal of Clinical Microbiology, October 2004, p. 4840-4842, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4840-4842.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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