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Journal of Clinical Microbiology, October 2004, p. 4843-4845, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4843-4845.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Fluorescent Amplified Fragment Length Polymorphism Subtyping of Multiresistant Salmonella enterica Serovar Typhimurium DT104

Andrew J. Lawson,1 John Stanley,2 E. John Threlfall,1 and Meeta Desai2*

Laboratory of Enteric Pathogens,1 Virus Reference Division, Specialist and Reference Microbiology Division, Health Protection Agency, London, United Kingdom2

Received 16 March 2004/ Returned for modification 22 April 2004/ Accepted 8 June 2004

Fluorescent amplified fragment length polymorphism (FAFLP) subtyping analysis was used to genotype multiresistant Salmonella enterica serovar Typhimurium definitive phage type 104. Thirteen distinct FAFLP profiles were found among 85 isolates exhibiting identical pulsed-field gel electrophoresis (PFGE) profiles. A single FAFLP profile was shared by 93% of outbreak-associated isolates and 82% of sporadic isolates. This study demonstrates the value of FAFLP as a high-resolution tool for epidemiological investigation of Salmonella.


* Corresponding author. Present address: Genomics, Proteomics and Bioinformatics Unit, Specialist and Reference Microbiology Division, Health Protection Agency, London NW9 5HT, United Kingdom. Phone: 44 (0)20 83276469. Fax: 44 (0)20 83276768. E-mail: meeta.desai{at}hpa.org.uk.


Journal of Clinical Microbiology, October 2004, p. 4843-4845, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4843-4845.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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