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Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

Hopital Debrousse, Hospices Civils de Lyon, Lyon,¹ Centre Hospitalier Universitaire, Grenoble, France,³ Freeman Hospital, Newcastle upon Tyne, United Kingdom,² Division of Clinical Microbiology, Institute of Hygiene and Medical Microbiology, Medical University of Vienna, Vienna, Austria,⁴ Universita degli Studi di Milano, Milan, Italy,⁵ Hospital Universitario La Fe, Valencia, Spain,⁶ Department of Medical Microbiology, University Medical Center, Nijmegen, The Netherlands⁷

Received 30 March 2004/ Returned for modification 10 May 2004/ Accepted 6 July 2004

When evaluated in six clinical laboratories from six countries with 1,174 fresh isolates, including 715 Candida glabrata and 459 non-C. glabrata strains, GLABRATA RTT (Fumouze Diagnostics, Levallois Perret, France) yielded an overall sensitivity and an overall specificity of 95.8 and 98.9%, respectively. The results were consistent from one laboratory to another. The five false-positive results corresponded to C. parapsilosis (n = 2), C. tropicalis, C. guilliermondii, and C. lusitaniae. GLABRATA RTT allows a rapid, cost-effective, and reliable presumptive identification of C. glabrata.