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Journal of Clinical Microbiology, October 2004, p. 4879-4881, Vol. 42, No. 10
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.10.4879-4881.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multilocus Variable-Number Tandem-Repeat Polymorphism among Brazilian Enterococcus faecalis Strains

Molecular Microbiology and Biotechnology,¹ Infection Control Sector, Universitarian Hospital of Brasília,⁴ Molecular Biology Laboratory, Institute of Biological Sciences, University of Brasília, Brasília,⁷ Bacteriology Division, Adolfo Lutz Institute, São Paulo, Brazil,⁶ Research Laboratory of Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven,² Erasmus MC, Department of Medical Microbiology & Infectious Diseases, Rotterdam,⁵ Division of Acute Internal Medicine and Infectious Diseases, Department of Internal Medicine, University Medical Center Utrecht, Utrecht, The Netherlands³

Received 29 March 2004/ Returned for modification 30 June 2004/ Accepted 6 July 2004

Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative to PFGE.

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