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Journal of Clinical Microbiology, November 2004, p. 4956-4960, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.4956-4960.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis

Agnes Marot-Leblond,1* Linda Grimaud,1 Sandrine David,2 Derek J. Sullivan,3 David C. Coleman,3 Jose Ponton,4 and Raymond Robert1

Groupe d'Etude des Interactions Hôte-Parasite, UPRES EA 3142, UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, Angers,1 SR2B, ZI Carrière Beurrière, Avrillé, France,2 Department of Oral Surgery, Oral Medicine and Oral Pathology, School of Dental Science, Trinity College, University of Dublin, Dublin, Republic of Ireland,3 Departementado de Immunologia, Microbiologia y Pathologia, Facultad de Medicina y Odontologia, Universidad del Pais, Vasco, Spain4

Received 18 December 2003/ Returned for modification 18 February 2004/ Accepted 17 July 2004

Candida dubliniensis was first established as a novel yeast species in 1995. It is particularly associated with recurrent episodes of oral candidosis in human immunodeficiency virus (HIV)-infected patients, but it has also been detected at other anatomical sites and at a low incidence level in non-HIV-infected patients. It shares so many phenotypic characteristics with C. albicans that it is easily misidentified as such. No rapid, simple, and commercial test that allows differentiation between C. dubliniensis and C. albicans has been developed, until now. Accurate species identification requires the use of genotype-based techniques that are not routinely available in most clinical microbiology diagnostic laboratories. The present study was designed to evaluate the efficiency of a new test (the immunochromatographic membrane [ICM] albi-dubli test; SR2B, Avrillé, France) to differentiate between C. albicans and C. dubliniensis. The organisms evaluated were strains whose identities had previously been confirmed by PCR tests and freshly isolated clinical strains and included 58 C. albicans isolates, 60 C. dubliniensis isolates, and 82 isolates belonging to other species of yeast. The ICM albi-dubli test is based on the principle of immunochromatographic analysis and involves the use of two distinct monoclonal antibodies that recognize two unrelated epitopes expressed by both species or specific to only one species. The assay requires no complex instrumentation for analysis and can be recommended for routine use in clinical microbiology laboratories. Results are obtained within 2 h and 30 min and are easy to interpret. This evaluation demonstrated the good performance of this immunochromatographic test for C. albicans and C. dubliniensis isolated on Sabouraud dextrose agar, CHOROMagar Candida, and CandidaSelect, with sensitivities and specificities ranging from 93.1 to 100%. These parameters decreased, however, to 91.4% when the test was performed with yeast isolated with Candida ID.

* Corresponding author. Mailing address: Laboratoire de Mycologie, Faculté de Pharmacie, 16 boulevard Daviers, 49 100 Angers, France. Phone: (33) 02 41 22 66 60. Fax: (33) 02 41 48 67 33. E-mail: agnes.marot{at}univ-angers.fr.

Journal of Clinical Microbiology, November 2004, p. 4956-4960, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.4956-4960.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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