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Journal of Clinical Microbiology, November 2004, p. 5001-5006, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5001-5006.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Application of Mycobacterial Interspersed Repetitive Unit Typing to Manitoba Tuberculosis Cases: Can Restriction Fragment Length Polymorphism Be Forgotten?

K. S. Blackwood,1* J. N. Wolfe,1 and A. M. Kabani1,2

National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada,1 Clinical Microbiology, Health Sciences Center, Winnipeg, Manitoba, Canada2

Received 22 April 2004/ Returned for modification 18 June 2004/ Accepted 4 August 2004

Since 1993, all Mycobacterium tuberculosis isolates recovered in the province of Manitoba, Canada, have been genotyped by the standard IS6110-restriction fragment length polymorphism (RFLP) method for routine surveillance, prevention, and control purposes. To date, our laboratory has collected 1,290 isolates, from which we have identified approximately 390 unique fingerprint patterns or "types." Although the standard method is well known for being a lengthy and labor-intensive procedure, a more efficient alternative for typing tuberculosis isolates, the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method, has recently gained acceptance. Consequently, all isolates acquired in 2003 (n = 126) were typed by both methods in order to determine the utility of replacing the RFLP method with MIRU typing for all future isolates. Application of Hunter's discriminatory index to the available study population showed that the MIRU method was close in discriminatory power (D) to the RFLP method (DMIRU = 0.831 to 0.984 versus DRFLP = 0.821 to 0.997). Clustering of isolates by using MIRU data correlated with RFLP-derived clustering, lending useful information for either an investigation or confirmation of an incidence of recent transmission. In addition, it was determined that each predominant RFLP type in Manitoba had a corresponding, recognizable MIRU type. It is conceivable that in the future RFLP typing can be replaced with MIRU for real-time, ongoing tuberculosis surveillance in the province.

* Corresponding author. Mailing address: National Reference Centre for Mycobacteriology, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba R3E 3R2, Canada. Phone: (204) 789-6039. Fax: (204) 789-2036. E-mail: kym_blackwood{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, November 2004, p. 5001-5006, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5001-5006.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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