This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nascimento, E. Articles by Goldman, G. H. Search for Related Content PubMed PubMed Citation Articles by Nascimento, E. Articles by Goldman, G. H.

Detection and Selection of Microsatellites in the Genome of Paracoccidioides brasiliensis as Molecular Markers for Clinical and Epidemiological Studies

Faculdade de Ciências Farmacêuticas de Ribeirão Preto,¹ Faculdade de Medicina de Ribeirão Preto,² Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo,⁴ Universidade do Vale do Paraíba, UNIVAP, Vale do Paraíba, Brazil,³ Department of Plant and Microbial Biology, University of California, Berkeley, California,⁵ Corporacion para Investigaciones Biológicas and Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia⁶

Received 5 April 2004/ Returned for modification 13 June 2004/ Accepted 5 July 2004

Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.

This article has been cited by other articles:

• Matute, D. R., Sepulveda, V. E., Quesada, L. M., Goldman, G. H., Taylor, J. W., Restrepo, A., McEwen, J. G. (2006). Microsatellite Analysis of Three Phylogenetic Species of Paracoccidioides brasiliensis.. J. Clin. Microbiol. 44: 2153-2157 [Abstract] [Full Text]