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Journal of Clinical Microbiology, November 2004, p. 5022-5028, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5022-5028.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Multilocus Variable-Number Tandem-Repeat Analysis for Typing Mycobacterium avium subsp. paratuberculosis

Pieter Overduin,1 Leo Schouls,2 Paul Roholl,3 Adri van der Zanden,4 Nofel Mahmmod,4 Arnold Herrewegh,1 and Dick van Soolingen1*

Mycobacteria Reference Laboratory,1 Laboratory of Toxicology, Pathology, and Genetics,3 Laboratory for National Vaccination Program Control, National Institute of Public Health and the Environment, Bilthoven,2 Medical Microbiology and Infectious Diseases and Gastroenterology Department, Gelre Hospital, Apeldoorn, The Netherlands4

Received 13 February 2004/ Returned for modification 29 March 2004/ Accepted 2 August 2004

The etiology of Crohn's disease in humans is largely unknown. Clinical signs of Crohn's disease partly resemble the clinical picture of Johne's disease in ruminants caused by Mycobacterium avium subsp. paratuberculosis. Because of the high prevalence of these bacteria in (products of) ruminants and their remarkable thermostability, concern has been raised about the possible role of these bacteria in the pathogenesis of Crohn's disease. In an attempt to develop a molecular typing method to facilitate meaningful comparative DNA fingerprinting of M. avium subsp. paratuberculosis isolates from the human and animal reservoirs, multilocus variable-number tandem-repeat analysis (MLVA) was explored and compared to IS900 restriction fragment length polymorphism (RFLP) typing. MLVA typing subdivided the most predominant RFLP type, R01, into six subtypes and thus provides a promising molecular subtyping approach to study the diversity of M. avium subsp. paratuberculosis.

* Corresponding author. Mailing address: National Institute of Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31 30 2742363. Fax: 31 30 2744418. E-mail: d.van.soolingen{at}rivm.nl.

Journal of Clinical Microbiology, November 2004, p. 5022-5028, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5022-5028.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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