Previous Article | Next Article 
Journal of Clinical Microbiology, November 2004, p. 5125-5132, Vol. 42, No. 11
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.11.5125-5132.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Detection of Campylobacter spp. in Chicken Fecal Samples by Real-Time PCR
Marianne Lund,*
Steen Nordentoft,
Karl Pedersen, and
Mogens Madsen
Danish
Institute for Food and Veterinary Research, Århus,
Denmark
Received 7 April 2004/
Returned for modification 9 May 2004/
Accepted 5 July 2004
A
real-time PCR assay for detecting thermophilic Campylobacter
spp. directly in chicken feces has been developed. DNA was isolated
from fecal material by using magnetic beads followed by PCR with a
prealiquoted PCR mixture, which had been stored at
18°C. Campylobacter could be detected in less
than 4 h, with a detection limit of 100 to 150 CFU/ml, in a
fecal suspension. A bacterial internal control was added before DNA
extraction to control both DNA isolation and the presence of PCR
inhibitors in the samples. The assay was performed on 111 swab samples
from a Danish surveillance program and compared to conventional
culturing using selective enrichment. There was no statistically
significant difference in performance between real-time PCR and culture
by selective enrichment, and the diagnostic specificity was 0.96 with
an agreement of 0.92. Therefore, the assay should be useful for
screening poultry flocks for the presence of
Campylobacter.
* Corresponding
author. Mailing address: Danish Institute for Food and Veterinary
Research (DFVF), Hangovej 2, DK 8200 Århus N, Denmark.
Phone: 45 78346878. Fax: 45 78346901. E-mail:
mlu{at}dfvf.dk.
Journal of Clinical Microbiology, November 2004, p. 5125-5132, Vol. 42, No. 11
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.11.5125-5132.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Brooks, J. P., McLaughlin, M. R.
(2009). Antibiotic Resistant Bacterial Profiles of Anaerobic Swine Lagoon Effluent. J. Environ. Qual.
38: 2431-2437
[Abstract]
[Full Text]
-
Olsen, K. N., Lund, M., Skov, J., Christensen, L. S., Hoorfar, J.
(2009). Detection of Campylobacter Bacteria in Air Samples for Continuous Real-Time Monitoring of Campylobacter Colonization in Broiler Flocks. Appl. Environ. Microbiol.
75: 2074-2078
[Abstract]
[Full Text]
-
Vereen, E. Jr., Lowrance, R. R., Cole, D. J., Lipp, E. K.
(2007). Distribution and Ecology of Campylobacters in Coastal Plain Streams (Georgia, United States of America). Appl. Environ. Microbiol.
73: 1395-1403
[Abstract]
[Full Text]
-
Krause, M., Josefsen, M. H., Lund, M., Jacobsen, N. R., Brorsen, L., Moos, M., Stockmarr, A., Hoorfar, J.
(2006). Comparative, Collaborative, and On-Site Validation of a TaqMan PCR Method as a Tool for Certified Production of Fresh, Campylobacter-Free Chickens. Appl. Environ. Microbiol.
72: 5463-5468
[Abstract]
[Full Text]
-
Ishii, S., Yan, T., Shively, D. A., Byappanahalli, M. N., Whitman, R. L., Sadowsky, M. J.
(2006). Cladophora (Chlorophyta) spp. Harbor Human Bacterial Pathogens in Nearshore Water of Lake Michigan.. Appl. Environ. Microbiol.
72: 4545-4553
[Abstract]
[Full Text]