Previous Article | Next Article 
Journal of Clinical Microbiology, November 2004, p. 5229-5237, Vol. 42, No. 11
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.11.5229-5237.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Type III Secretion Phenotypes of Pseudomonas aeruginosa Strains Change during Infection of Individuals with Cystic Fibrosis
Manu Jain,1
Daniel Ramirez,2
Roopa Seshadri,3,4
Joanne F. Cullina,1
Catherine A. Powers,5
Grant S. Schulert,2,
Maskit Bar-Meir,6
Christine L. Sullivan,4
Susanna A. McColley,5 and
Alan R. Hauser1,2*
Department of Medicine,1
Department of Microbiology/Immunology,2
Departments of Pediatrics and Preventive Medicine, Northwestern University,3
Child Health Research Program, Children's Memorial Institute for Education and Research,4
Division of Pulmonary and Critical Care Medicine,5
Division of Infectious Diseases, Children's Memorial Hospital, Chicago, Illinois6
Received 18 March 2004/
Returned for modification 14 June 2004/
Accepted 30 July 2004
Pseudomonas aeruginosa is a frequent cause of respiratory exacerbations in individuals with cystic fibrosis. An important virulence determinant of this pathogen is its type III protein secretion system. In this study, the type III secretion properties of 435 P. aeruginosa respiratory isolates from 56 chronically infected individuals with cystic fibrosis were investigated. Although it had been previously reported that 75 to 90% of P. aeruginosa isolates from patients with hospital-acquired pneumonia secreted type III proteins, only 12% of isolates from cystic fibrosis patients did so, with nearly all of these isolates secreting ExoS and ExoT but not ExoU. Despite the low overall prevalence of type III protein-secreting isolates, at least one secreting isolate was cultured from one-third of cystic fibrosis patients. Interestingly, the fraction of cystic fibrosis patient isolates capable of secreting type III proteins decreased with duration of infection. Although 90% of isolates from the environment, the presumed reservoir for the majority of P. aeruginosa strains that infect patients with cystic fibrosis, secreted type III proteins, only 49% of isolates from newly infected children, 18% of isolates from chronically infected children, and 4% of isolates from chronically infected adults with cystic fibrosis secreted these proteins. Within individual patients, isolates of clonal origin differed in their secretion phenotypes, indicating that as strains persisted in cystic fibrosis patient airways, their type III protein secretion properties changed. Together, these findings indicate that following infection of cystic fibrosis patient airways, P. aeruginosa strains gradually change from a type III protein secretion-positive phenotype to a secretion-negative phenotype.
* Corresponding author. Mailing address: Northwestern University, 303 East Chicago Ave., Searle 6-495, Chicago, IL 60611. Phone: (312) 503-1044. Fax: (312) 503-1339. E-mail:
ahauser{at}northwestern.edu.
Present address: University of Iowa, Iowa City, Iowa.
Journal of Clinical Microbiology, November 2004, p. 5229-5237, Vol. 42, No. 11
0095-1137/04/$08.00+0 DOI: 10.1128/JCM.42.11.5229-5237.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Foweraker, J. E., Laughton, C. R., Brown, D. F., Bilton, D.
(2009). Comparison of Methods To Test Antibiotic Combinations against Heterogeneous Populations of Multiresistant Pseudomonas aeruginosa from Patients with Acute Infective Exacerbations in Cystic Fibrosis. Antimicrob. Agents Chemother.
53: 4809-4815
[Abstract]
[Full Text]
-
Kim, H.-S., Ma, B., Perna, N. T., Charkowski, A. O.
(2009). Phylogeny and Virulence of Naturally Occurring Type III Secretion System-Deficient Pectobacterium Strains. Appl. Environ. Microbiol.
75: 4539-4549
[Abstract]
[Full Text]
-
Baer, M., Sawa, T., Flynn, P., Luehrsen, K., Martinez, D., Wiener-Kronish, J. P., Yarranton, G., Bebbington, C.
(2009). An Engineered Human Antibody Fab Fragment Specific for Pseudomonas aeruginosa PcrV Antigen Has Potent Antibacterial Activity. Infect. Immun.
77: 1083-1090
[Abstract]
[Full Text]
-
Goodman, A. L., Merighi, M., Hyodo, M., Ventre, I., Filloux, A., Lory, S.
(2009). Direct interaction between sensor kinase proteins mediates acute and chronic disease phenotypes in a bacterial pathogen. Genes Dev.
23: 249-259
[Abstract]
[Full Text]
-
Choy, M. H., Stapleton, F., Willcox, M. D. P., Zhu, H.
(2008). Comparison of virulence factors in Pseudomonas aeruginosa strains isolated from contact lens- and non-contact lens-related keratitis. J Med Microbiol
57: 1539-1546
[Abstract]
[Full Text]
-
El Solh, A. A., Akinnusi, M. E., Wiener-Kronish, J. P., Lynch, S. V., Pineda, L. A., Szarpa, K.
(2008). Persistent Infection with Pseudomonas aeruginosa in Ventilator-associated Pneumonia. Am. J. Respir. Crit. Care Med.
178: 513-519
[Abstract]
[Full Text]
-
Willcox, M. D. P., Zhu, H., Conibear, T. C. R., Hume, E. B. H., Givskov, M., Kjelleberg, S., Rice, S. A.
(2008). Role of quorum sensing by Pseudomonas aeruginosa in microbial keratitis and cystic fibrosis. Microbiology
154: 2184-2194
[Abstract]
[Full Text]
-
Shen, D.-K., Filopon, D., Chaker, H., Boullanger, S., Derouazi, M., Polack, B., Toussaint, B.
(2008). High-cell-density regulation of the Pseudomonas aeruginosa type III secretion system: implications for tryptophan catabolites. Microbiology
154: 2195-2208
[Abstract]
[Full Text]
-
Yang, L., Haagensen, J. A. J., Jelsbak, L., Johansen, H. K., Sternberg, C., Hoiby, N., Molin, S.
(2008). In Situ Growth Rates and Biofilm Development of Pseudomonas aeruginosa Populations in Chronic Lung Infections. J. Bacteriol.
190: 2767-2776
[Abstract]
[Full Text]
-
Miao, E. A., Ernst, R. K., Dors, M., Mao, D. P., Aderem, A.
(2008). Pseudomonas aeruginosa activates caspase 1 through Ipaf. Proc. Natl. Acad. Sci. USA
105: 2562-2567
[Abstract]
[Full Text]
-
Mulcahy, H., O'Callaghan, J., O'Grady, E. P., Macia, M. D., Borrell, N., Gomez, C., Casey, P. G., Hill, C., Adams, C., Gahan, C. G. M., Oliver, A., O'Gara, F.
(2008). Pseudomonas aeruginosa RsmA Plays an Important Role during Murine Infection by Influencing Colonization, Virulence, Persistence, and Pulmonary Inflammation. Infect. Immun.
76: 632-638
[Abstract]
[Full Text]
-
Tingpej, P., Smith, L., Rose, B., Zhu, H., Conibear, T., Al Nassafi, K., Manos, J., Elkins, M., Bye, P., Willcox, M., Bell, S., Wainwright, C., Harbour, C.
(2007). Phenotypic Characterization of Clonal and Nonclonal Pseudomonas aeruginosa Strains Isolated from Lungs of Adults with Cystic Fibrosis. J. Clin. Microbiol.
45: 1697-1704
[Abstract]
[Full Text]
-
Smith, L., Rose, B., Tingpej, P., Zhu, H., Conibear, T., Manos, J., Bye, P., Elkins, M., Willcox, M., Bell, S., Wainwright, C., Harbour, C.
(2006). Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis.. J Med Microbiol
55: 1641-1644
[Abstract]
[Full Text]
-
Nguyen, D., Singh, P. K.
(2006). Evolving stealth: Genetic adaptation of Pseudomonas aeruginosa during cystic fibrosis infections. Proc. Natl. Acad. Sci. USA
103: 8305-8306
[Full Text]
-
Aspedon, A., Palmer, K., Whiteley, M.
(2006). Microarray Analysis of the Osmotic Stress Response in Pseudomonas aeruginosa.. J. Bacteriol.
188: 2721-2725
[Abstract]
[Full Text]
-
Ventre, I., Goodman, A. L., Vallet-Gely, I., Vasseur, P., Soscia, C., Molin, S., Bleves, S., Lazdunski, A., Lory, S., Filloux, A.
(2006). Multiple sensors control reciprocal expression of Pseudomonas aeruginosa regulatory RNA and virulence genes. Proc. Natl. Acad. Sci. USA
103: 171-176
[Abstract]
[Full Text]
-
Shaver, C. M., Hauser, A. R.
(2006). Interactions between effector proteins of the Pseudomonas aeruginosa type III secretion system do not significantly affect several measures of disease severity in mammals. Microbiology
152: 143-152
[Abstract]
[Full Text]
-
Corech, R., Rao, A., Laxova, A., Moss, J., Rock, M. J., Li, Z., Kosorok, M. R., Splaingard, M. L., Farrell, P. M., Barbieri, J. T.
(2005). Early Immune Response to the Components of the Type III System of Pseudomonas aeruginosa in Children with Cystic Fibrosis. J. Clin. Microbiol.
43: 3956-3962
[Abstract]
[Full Text]
-
Foweraker, J. E., Laughton, C. R., Brown, D. F. J., Bilton, D.
(2005). Phenotypic variability of Pseudomonas aeruginosa in sputa from patients with acute infective exacerbation of cystic fibrosis and its impact on the validity of antimicrobial susceptibility testing. J Antimicrob Chemother
55: 921-927
[Abstract]
[Full Text]