This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nadkarni, M. A.
Right arrow Articles by Hunter, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nadkarni, M. A.
Right arrow Articles by Hunter, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2004, p. 5238-5244, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5238-5244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Carious Dentine Provides a Habitat for a Complex Array of Novel Prevotella-Like Bacteria

Mangala A. Nadkarni,1* C. Elizabeth Caldon,1 Kim-Ly Chhour,1 Ilana P. Fisher,1 F. Elizabeth Martin,1,2 Nicholas A. Jacques,1 and Neil Hunter1

Institute of Dental Research, Westmead Millennium Institute, and Westmead Centre for Oral Health, Westmead,1 Faculty of Dentistry, The University of Sydney, Sydney, New South Wales, Australia2

Received 5 May 2004/ Returned for modification 17 June 2004/ Accepted 15 July 2004

Previous analysis of the microbiology of advanced caries by culture and real-time PCR emphasized the high incidence and abundance of gram-negative anaerobic species, particularly Prevotella-like bacteria. The diversity of Prevotella-like bacteria was further explored by analyzing pooled bacterial DNA from lesions of carious dentine. This was achieved by amplification of a region of the 16S ribosomal DNA with a Prevotella genus-specific forward primer and a universal bacterial reverse primer, followed by cloning and sequencing. Cultured Prevotella species commonly associated with oral tissues constituted only 12% of the Prevotella clones isolated from advanced carious lesions. The remaining 88% consisted of a diverse range of phylotypes. These included five clusters of previously recognized but uncultured oral Prevotella spp. and a major cluster containing Prevotella-like bacteria most closely related to uncharacterized rumen bacteria. Cluster-specific primers were designed, and the numbers of bacteria within clusters were quantified by real-time PCR, confirming the abundance of these organisms. The data indicated that advanced dental caries provides a unique environment for a complex array of novel and uncultured Prevotella and Prevotella-like bacteria which, in some cases, may dominate the diverse polymicrobial community associated with the disease.

* Corresponding author. Mailing address: Institute of Dental Research, Westmead Centre for Oral Health, P.O. Box 533, Wentworthville, NSW 2145, Australia. Phone: 61-2-9845-7826. Fax: 61-2-9845-7599. E-mail: mnadkarni{at}dental.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, November 2004, p. 5238-5244, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5238-5244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Aas, J. A., Griffen, A. L., Dardis, S. R., Lee, A. M., Olsen, I., Dewhirst, F. E., Leys, E. J., Paster, B. J. (2008). Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults. J. Clin. Microbiol. 46: 1407-1417 [Abstract] [Full Text]  
  • Downes, J., Sutcliffe, I. C., Booth, V., Wade, W. G. (2007). Prevotella maculosa sp. nov., isolated from the human oral cavity. Int. J. Syst. Evol. Microbiol. 57: 2936-2939 [Abstract] [Full Text]  
  • Sakamoto, M., Umeda, M., Ishikawa, I., Benno, Y. (2005). Prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. Int. J. Syst. Evol. Microbiol. 55: 1839-1843 [Abstract] [Full Text]  
  • Chhour, K.-L., Nadkarni, M. A., Byun, R., Martin, F. E., Jacques, N. A., Hunter, N. (2005). Molecular Analysis of Microbial Diversity in Advanced Caries. J. Clin. Microbiol. 43: 843-849 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»