This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nishio, H. Articles by Arakawa, Y. Search for Related Content PubMed PubMed Citation Articles by Nishio, H. Articles by Arakawa, Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2004, p. 5256-5263, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5256-5263.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Metallo-ß-Lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan

Clinical Laboratory, Shiga Medical Center for Adults,¹ Clinical Laboratory, Social Insurance Shiga Hospital, Shiga,⁴ Department of Clinical Pathology, Tenri Hospital, Nara,² Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo,³ Department of Clinical and Laboratory Medicine, National Cardiovascular Center,⁵ Department of Medical Technology, Kinki University School of Medicine,⁶ Laboratory for Clinical Investigation, Osaka University Hospital,⁷ Clinical Central Laboratory, Kansai Medical University Hospital,⁸ Division of Biomedical Informatics, Course of Health Science, Graduate School of Medicine, Osaka University,¹³ Clinical Laboratory, The Research Foundation for Microbial Diseases of Osaka University, Osaka,¹⁴ Clinical Laboratory, Hyogo Medical University Hospital,⁹ Clinical Laboratory, Takarazuka Municipal Hospital,¹⁰ Clinical Laboratory, Hyogo Prefectural Amagasaki Hospital,¹¹ Clinical Laboratory, Kobe University Hospital, Hyogo,¹⁵ Clinical Laboratory, Wakayama Rosai Hospital, Wakayama, Japan,¹²

Received 11 March 2004/ Returned for modification 1 June 2004/ Accepted 19 July 2004

A total of 19,753 strains of gram-negative rods collected during two 6-month periods (October 2000 to March 2001 and November 2001 to April 2002) from 13 clinical laboratories in the Kinki region of Japan were investigated for the production of metallo-ß-lactamases (MBLs). MBLs were detected in 96 (0.5%) of the 19,753 isolates by the broth microdilution method, the 2-mercaptopropionic acid inhibition test, and PCR and DNA sequencing analyses. MBL-positive isolates were detected in 9 of 13 laboratories, with the rate of detection ranging between 0 and 2.6% for each laboratory. Forty-four of 1,429 (3.1%) Serratia marcescens, 22 of 6,198 (0.4%) Pseudomonas aeruginosa, 21 of 1,108 (1.9%) Acinetobacter spp., 4 of 544 (0.7%) Citrobacter freundii, 3 of 127 (2.4%) Providencia rettgeri, 1 of 434 (0.2%) Morganella morganii, and 1 of 1,483 (0.1%) Enterobacter cloacae isolates were positive for MBLs. Of these 96 MBL-positive strains, 87 (90.6%), 7 (7.3%), and 2 (2.1%) isolates carried the genes for IMP-1-group MBLs, IMP-2-group MBLs, and VIM-2-group MBLs, respectively. The class 1 integrase gene, intI1, was detected in all MBL-positive strains, and the aac (6')-Ib gene was detected in 37 (38.5%) isolates. Strains with identical PCR fingerprint profiles in a random amplified polymorphic DNA pattern analysis were isolated successively from five separate hospitals, suggesting the nosocomial spread of the organism in each hospital. In conclusion, many species of MBL-positive gram-negative rods are distributed widely in different hospitals in the Kinki region of Japan. The present findings should be considered during the development of policies and strategies to prevent the emergence and further spread of MBL-producing bacteria.

This article has been cited by other articles:

• Peleg, A. Y., Seifert, H., Paterson, D. L. (2008). Acinetobacter baumannii: Emergence of a Successful Pathogen. Clin. Microbiol. Rev. 21: 538-582 [Abstract] [Full Text]  
• Biendo, M., Canarelli, B., Thomas, D., Rousseau, F., Hamdad, F., Adjide, C., Laurans, G., Eb, F. (2008). Successive Emergence of Extended-Spectrum {beta}-Lactamase-Producing and Carbapenemase-Producing Enterobacter aerogenes Isolates in a University Hospital. J. Clin. Microbiol. 46: 1037-1044 [Abstract] [Full Text]  
• Perez, F., Hujer, A. M., Hujer, K. M., Decker, B. K., Rather, P. N., Bonomo, R. A. (2007). Global Challenge of Multidrug-Resistant Acinetobacter baumannii. Antimicrob. Agents Chemother. 51: 3471-3484 [Full Text]  
• Queenan, A. M., Bush, K. (2007). Carbapenemases: the Versatile {beta}-Lactamases. Clin. Microbiol. Rev. 20: 440-458 [Abstract] [Full Text]  
• Tsakris, A., Ikonomidis, A., Spanakis, N., Poulou, A., Pournaras, S. (2007). Characterization of In3Mor, a new integron carrying VIM-1 metallo-{beta}-lactamase and sat1 gene, from Morganella morganii. J Antimicrob Chemother 59: 739-741 [Abstract] [Full Text]  
• Yong, D., Choi, Y. S., Roh, K. H., Kim, C. K., Park, Y. H., Yum, J. H., Lee, K., Chong, Y. (2006). Increasing Prevalence and Diversity of Metallo-{beta}-Lactamases in Pseudomonas spp., Acinetobacter spp., and Enterobacteriaceae from Korea.. Antimicrob. Agents Chemother. 50: 1884-1886 [Abstract] [Full Text]