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Journal of Clinical Microbiology, November 2004, p. 5324-5326, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5324-5326.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prevalence and Characterization of Leukotoxin-Producing Staphylococcus intermedius in Isolates from Dogs and Pigeons

Keiko Futagawa-Saito,1* Tsukasa Sugiyama,1 Sayaka Karube,1 Naomi Sakurai,2 William Ba-Thein,3 and Tsuguaki Fukuyasu1

Center for Medical Sciences, School of Health Sciences, Ibaraki Prefectural University of Health Sciences, Inashiki,2 Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki,3 Department of Animal Health 2, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan1

Received 22 March 2004/ Returned for modification 29 May 2004/ Accepted 25 July 2004

Staphylococcus intermedius isolates from dogs (n = 44) and pigeons (n = 62) were categorized into 12 types by intergenic ribosomal DNA spacer polymorphism analysis. All isolates from pigeons were lukS positive and all isolates from dogs were lukS and lukF positive by dot blot analysis. The mean leukotoxicity titer for dog isolates was at least 129-fold higher than that for pigeon isolates.

* Corresponding author. Mailing address: Department of Animal Health 2, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan. Phone: 81 (42) 754 7111. Fax: 81 (42) 768 2588. E-mail: saitohk{at}azabu-u.ac.jp.

Journal of Clinical Microbiology, November 2004, p. 5324-5326, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5324-5326.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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