This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cloud, J. L.
Right arrow Articles by Woods, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cloud, J. L.
Right arrow Articles by Woods, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2004, p. 5341-5344, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5341-5344.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of a Modified Gen-Probe Amplified Direct Test for Detection of Mycobacterium tuberculosis Complex Organisms in Cerebrospinal Fluid

Joann L. Cloud,1* Cheryl Shutt,1 Wade Aldous,1,2 and Gail Woods1,2

ARUP Institute for Clinical and Experimental Pathology,1 Department of Pathology, University of Utah, Salt Lake City, Utah2

Received 14 April 2004/ Returned for modification 10 June 2004/ Accepted 27 July 2004

Laboratory evidence for tuberculous meningitis is difficult to acquire due to the low numbers of organisms present in cerebrospinal fluid (CSF) and the presence of nucleic acid amplification inhibitors. The Amplified Mycobacterium tuberculosis Direct Test (MTD) is sensitive and specific for the direct detection of M. tuberculosis complex in respiratory samples but has not been approved for CSF. We evaluated a modified version of the current MTD, optimized for use with CSF samples. Samples were prepared by spiking CSF with various numbers of M. tuberculosis complex organisms. The modified MTD performance was compared with results obtained using a purified RNA sample extracted using the Qiagen RNeasy Protect Bacteria Mini Kit. By use of CSF artificially spiked with M. tuberculosis complex, the sensitivity of the modified MTD was 100% (six of six) for CSF samples containing approximately 600 CFU/ml, 78% (seven of nine) for approximately 60 CFU/ml, 50% (three of six) for 6 CFU/ml, and 17% (one of six) for samples with <1 CFU/ml. The specificity of the modified MTD method was 100% (22 of 22). The sensitivity of the Qiagen MTD method was 100% for CSF samples containing approximately 600 CFU/ml (six of six) and approximately 60 CFU/ml (nine of nine), 50% for samples with approximately 6 CFU/ml (three of six), and 50% for samples with <1 CFU/ml (three of six). The specificity of the Qiagen MTD method was 86% (19 of 22). With the Qiagen MTD method, however, initial results were equivocal for 14 of the 27 (52%) positive samples, requiring repeat analysis, whereas with the modified MTD, only 1 of 27 (4%) was equivocal. The modified MTD for CSF samples was less time-consuming and less expensive and resulted in considerably fewer equivocal results than the Qiagen MTD method did.

* Corresponding author. Mailing address: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787, ext. 2439. Fax: (801) 584-5207. E-mail: cloudjl{at}aruplab.com.

Journal of Clinical Microbiology, November 2004, p. 5341-5344, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5341-5344.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Piersimoni, C., Gherardi, G., Nista, D., Bornigia, S. (2009). Impact of a Chemistry-Based DNA Extraction Method on Performance of a Commercial Amplification Assay for Detection of Mycobacterium tuberculosis Complex. J. Clin. Microbiol. 47: 282-283 [Full Text]  
  • Rock, R. B., Olin, M., Baker, C. A., Molitor, T. W., Peterson, P. K. (2008). Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects. Clin. Microbiol. Rev. 21: 243-261 [Abstract] [Full Text]  
  • Boudewijns, M., Bakkers, J. M., Sturm, P. D. J., Melchers, W. J. G. (2006). 16S rRNA Gene Sequencing and the Routine Clinical Microbiology Laboratory: a Perfect Marriage?. J. Clin. Microbiol. 44: 3469-3470 [Full Text]  
  • Shah, N. P., Singhal, A., Jain, A., Kumar, P., Uppal, S. S., Srivatsava, M. V. P., Prasad, H. K. (2006). Occurrence of Overlooked Zoonotic Tuberculosis: Detection of Mycobacterium bovis in Human Cerebrospinal Fluid. J. Clin. Microbiol. 44: 1352-1358 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»