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Journal of Clinical Microbiology, November 2004, p. 5349-5352, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5349-5352.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sensitive and Specific Detection of Yersinia pseudotuberculosis by Loop-Mediated Isothermal Amplification

Tomoko Horisaka,1 Kayoko Fujita,1 Taketoshi Iwata,1 Aya Nakadai,1 Alexandre T. Okatani,2 Tetsuya Horikita,1 Takahide Taniguchi,1 Eiichi Honda,1 Yuichi Yokomizo,3 and Hideki Hayashidani1*

Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo,1 School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa,2 National Institute of Animal Health, Tsukuba, Ibaragi, Japan3

Received 8 March 2004/ Returned for modification 23 April 2004/ Accepted 5 July 2004

We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis.

* Corresponding author. Mailing address: Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan. Phone and Fax: 81-42-367-5775. E-mail: eisei{at}cc.tuat.ac.jp.

Journal of Clinical Microbiology, November 2004, p. 5349-5352, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5349-5352.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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