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Journal of Clinical Microbiology, November 2004, p. 5388-5391, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5388-5391.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain

Alicia Aranaz,* Beatriz Romero, Natalia Montero, Julio Álvarez, Javier Bezos, Lucía de Juan, Ana Mateos, and Lucas Domínguez

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

Received 23 February 2004/ Returned for modification 16 April 2004/ Accepted 23 June 2004

Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.

* Corresponding author. Mailing address: Departamento de Sanidad Animal, Facultad de Veterinaria, U.C.M. Av. Puerta de Hierro s/n, 28040 Madrid, Spain. Phone: 34 91 3944083. Fax: 34 91 3943908. E-mail: alaranaz{at}vet.ucm.es.

Journal of Clinical Microbiology, November 2004, p. 5388-5391, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5388-5391.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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