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Journal of Clinical Microbiology, November 2004, p. 5403-5405, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5403-5405.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

An Unexpected Experimental Pitfall in the Molecular Diagnosis of Bacterial Endophthalmitis

Luana Ugahary,1 Wendy van de Sande,2 Jan C. van Meurs,1 and Alex van Belkum2*

The Rotterdam Eye Hospital,1 Department of Medical Microbiology & Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands2

Received 2 June 2004/ Returned for modification 5 July 2004/ Accepted 17 July 2004

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

* Corresponding author. Mailing address: Erasmus MC, Department of Medical Microbiology & Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 00-31-10-4635813. Fax: 00-31-10-4633875. E-mail: a.vanbelkum{at}erasmusmc.nl.

Journal of Clinical Microbiology, November 2004, p. 5403-5405, Vol. 42, No. 11
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.11.5403-5405.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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