This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suzuki, M.
Right arrow Articles by Sakae, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, M.
Right arrow Articles by Sakae, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2004, p. 5462-5466, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5462-5466.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Rapid PCR Method Using the Insertion Sequence IS1203 for Genotyping Shiga Toxin-Producing Escherichia coli O157

Masahiro Suzuki,* Masakado Matsumoto, Mami Hata, Masao Takahashi, and Kenji Sakae

Department of Microbiology, Aichi Prefectural Institute of Public Health, Nagoya, Aichi, Japan

Received 7 May 2004/ Returned for modification 14 July 2004/ Accepted 13 August 2004

We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fingerprinting patterns produced from the upstream or downstream regions of IS1203 were obtained within 1 or 2 days. By combining the two patterns, 79 STEC O157 isolates were classified into 39 types, which were then classified into 36 subtypes by pulsed-field gel electrophoresis (PFGE). The discriminatory power of IS1203 PCR typing (D = 0.974) is similar to that of PFGE (D = 0.981). This method can be used for rapid and simplified genotyping.

* Corresponding author. Mailing address: 7-6 Nagare Tsuji-machi Kita-ku, Nagoya 462-8576, Aichi, Japan. Phone: 81-52-910-5669. Fax: 81-52-913-3641. E-mail: masahiro_4_suzuki{at}pref.aichi.lg.jp.

Journal of Clinical Microbiology, December 2004, p. 5462-5466, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5462-5466.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ooka, T., Terajima, J., Kusumoto, M., Iguchi, A., Kurokawa, K., Ogura, Y., Asadulghani, M., Nakayama, K., Murase, K., Ohnishi, M., Iyoda, S., Watanabe, H., Hayashi, T. (2009). Development of a Multiplex PCR-Based Rapid Typing Method for Enterohemorrhagic Escherichia coli O157 Strains. J. Clin. Microbiol. 47: 2888-2894 [Abstract] [Full Text]  
  • Hajra, T. K., Bag, P. K., Das, S. C., Mukherjee, S., Khan, A., Ramamurthy, T. (2007). Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC. CVI 14: 600-604 [Abstract] [Full Text]  
  • Singh, A., Goering, R. V., Simjee, S., Foley, S. L., Zervos, M. J. (2006). Application of Molecular Techniques to the Study of Hospital Infection. Clin. Microbiol. Rev. 19: 512-530 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»