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Journal of Clinical Microbiology, December 2004, p. 5542-5548, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5542-5548.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nicoletella semolina gen. nov., sp. nov., a New Member of Pasteurellaceae Isolated from Horses with Airway Disease

Peter Kuhnert,1* Bozena Korczak,1 Enevold Falsen,2 Reto Straub,3 Anneliese Hoops,4 Patrick Boerlin,1,{dagger} Joachim Frey,1 and Reinier Mutters4

Institute of Veterinary Bacteriology,1 Department of Equine Internal Medicine, University of Bern, Bern, Switzerland,3 Culture Collection of the University of Göteborg, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden,2 Institute for Medical Microbiology and Hygiene, Philipps University, Marburg, Germany4

Received 24 May 2004/ Returned for modification 5 July 2004/ Accepted 15 August 2004

Gram-negative, nonmotile bacteria that are catalase, oxidase, and urease positive are regularly isolated from the airways of horses with clinical signs of respiratory disease. On the basis of the findings by a polyphasic approach, we propose that these strains be classified as Nicoletella semolina gen. nov, sp. nov., a new member of the family Pasteurellaceae. N. semolina reduces nitrate to nitrite but is otherwise biochemically inert; this includes the lack of an ability to ferment glucose and other sugars. Growth is fastidious, and the isolates have a distinctive colony morphology, with the colonies being dry and waxy and looking like a semolina particle that can be moved around on an agar plate without losing their shape. DNA-DNA hybridization data and multilocus phylogenetic analysis, including 16S rRNA gene (rDNA), rpoB, and infB sequencing, clearly placed N. semolina as a new genus in the family Pasteurellaceae. In all the phylogenetic trees constructed, N. semolina is on a distinct branch displaying ~5% 16S rDNA, ~16% rpoB, and ~20% infB sequence divergence from its nearest relative within the family Pasteurellaceae. High degrees of conservation of the 16S rDNA (99.8%), rpoB (99.6%), and infB (99.7%) sequences exist within the species, indicating that N. semolina isolates not only are phenotypically homogeneous but also are genetically homogeneous. The type strain of N. semolina is CCUG43639T (DSM16380T).

* Corresponding author. Mailing address: Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland. Phone: 41-31-6312485. Fax: 41-31-6312634. E-mail: peter.kuhnert{at}vbi.unibe.ch.

{dagger} Present address: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Journal of Clinical Microbiology, December 2004, p. 5542-5548, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5542-5548.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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