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Journal of Clinical Microbiology, December 2004, p. 5565-5570, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5565-5570.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

"Bacteroides nordii" sp. nov. and "Bacteroides salyersae" sp. nov. Isolated from Clinical Specimens of Human Intestinal Origin

Y. L. Song,1* C. X. Liu,1 M. McTeague,2 and S. M. Finegold3,4,5

Research Service,1 Clinical Microbiology Laboratory,2 Infectious Diseases Section, VA Medical Center West Los Angeles,3 Department of Medicine,4 Department of Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, California5

Received 21 May 2004/ Returned for modification 12 July 2004/ Accepted 13 August 2004

Two groups of unknown bacteria, which phenotypically resemble members of the Bacteroides fragilis group but phylogenetically display >5% 16S rRNA gene sequence divergence from their nearest validly described species, Bacteroides thetaiotaomicron, were characterized by phenotypic and molecular taxonomic methods. Phylogenetically and phenotypically, the unidentified bacteria displayed a relatively close association with each other. However, a 16S rRNA gene sequence divergence of approximately 4% between the two unknown bacteria, as well as distinguishable biochemical characteristics, demonstrates that these organisms are genotypically and phenotypically distinct, and each group may represent a previously unknown subline within the Bacteroides phylogenetic cluster. Subsequent DNA-DNA hybridization studies confirmed that the two novel organisms were indeed distinct from each other. The previously described species closest to both of them is B. thetaiotaomicron (approximately 94% sequence similarity), but they can be differentiated easily from B. thetaiotaomicron by virtue of not utilizing trehalose. DNA-DNA pairing studies also documented the separateness of the unknown species and B. thetaiotaomicron. Based on the phenotypic and phylogenetic findings, two new species, "Bacteroides nordii" sp. nov. and "Bacteroides salyersae" sp. nov, are proposed. The G+C content of the DNA is 41.4 mol% for Bacteroides nordii and 42.0 mol% for Bacteroides salyersae. The type strains of Bacteroides nordii and Bacteroides salyersae are WAL 11050 (ATCC BAA-998 or CCUG 48943) and WAL 10018 (ATCC BAA-997 or CCUG 48945), respectively.

* Corresponding author. Mailing address: VA Medical Center WLA., Bldg. 304, Rm. E3-227, 11301 Wilshire Blvd., Los Angeles, CA 90073. Phone: (310) 478-3711, ext. 49151. Fax: (310) 268-4458. E-mail: yulis1{at}yahoo.com.

Journal of Clinical Microbiology, December 2004, p. 5565-5570, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5565-5570.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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