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Journal of Clinical Microbiology, December 2004, p. 5644-5649, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5644-5649.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Multilocus Sequence Typing Scheme for the Opportunistic Pathogen Pseudomonas aeruginosa

Barry Curran,1 Daniel Jonas,2 Hajo Grundmann,2 Tyrone Pitt,3 and Christopher G. Dowson1*

Department of Biological Sciences, University of Warwick, Coventry,1 Laboratory of Hospital Infection, Health Protection Agency, Colindale, London, United Kingdom,3 Institute of Environmental Medicine and Hospital Epidemiology, Freiburg University Hospital, Freiburg, Germany2

Received 14 June 2004/ Returned for modification 30 July 2004/ Accepted 23 August 2004

A multilocus sequence typing (MLST) scheme has been developed for Pseudomonas aeruginosa which provides molecular typing data that are highly discriminatory and electronically portable between laboratories. MLST data confirm the data from previous studies that suggest that P. aeruginosa is best described as nonclonal but as having an epidemic population. The index of association was 0.17, indicating a freely recombining population; however, there was evidence of clusters of closely related strains or clonal complexes among the members of this population. It is apparent that the sequence types (STs) from single isolates, representing each of the present epidemic clones in the United Kingdom from Liverpool, Manchester, and the West Midlands, are not closely related to each other. This suggests distinct evolutionary origins for each of these epidemic clones in the United Kingdom. Furthermore, these clones are distinct from European clone C. Comparison of the results of MLST with those of toxA typing and serotyping revealed that strains with identical STs may possess different toxA types and diverse serotypes. Given that recombination is important in the population of P. aeruginosa, the lack of a linkage between toxA type and serotype is not surprising and reveals the strength of the MLST approach for obtaining a better understanding of the epidemiology of P. aeruginosa.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: 44 2476 523534. Fax: 44 2476 523568. E-mail: c.g.dowson{at}warwick.ac.uk.

Journal of Clinical Microbiology, December 2004, p. 5644-5649, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5644-5649.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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