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Journal of Clinical Microbiology, December 2004, p. 5681-5688, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5681-5688.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Analysis of Diverse Disease-Causing Pneumococci Indicates High Levels of Diversity within Serotypes and Capsule Switching

Johanna M. C. Jefferies,1 Andrew Smith,2 Stuart C. Clarke,1,3 Chris Dowson,4 and Timothy J. Mitchell1*

Institute of Biomedical and Life Sciences, Division of Infection & Immunity, University of Glasgow,1 Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital,3 Infection & Immunity Group, Glasgow Dental School, Glasgow,2 Department of Biological Sciences, University of Warwick, Coventry, United Kingdom4

Received 2 July 2004/ Returned for modification 20 August 2004/ Accepted 30 August 2004

We have used multilocus sequence typing (MLST) and serotyping to build a phylogenetic framework for pneumococcal disease isolates in Scotland that provides a snapshot of the relationships between capsular type and genotype. The results show that while the MLST type correlates with the serotype, isolates within a serotype can belong to a number of individual clonal complexes or sequence types (STs). We also show that isolates of the same ST can express different capsular polysaccharides, i.e., display capsular switching, and that this phenomenon is observed both for capsular types commonly isolated from patients with invasive disease and for serogroups less commonly isolated from patients with invasive disease but which may commonly be carried asymptomatically in the human nasopharynx.

* Corresponding author. Mailing address: IBLS, Division of Infection & Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom. Phone: 0141 330 3479. Fax: 0141 330 3737. E-mail: T.Mitchell{at}bio.gla.ac.uk.

Journal of Clinical Microbiology, December 2004, p. 5681-5688, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5681-5688.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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