This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sakai, H. Articles by Bauer, T. W. Search for Related Content PubMed PubMed Citation Articles by Sakai, H. Articles by Bauer, T. W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2004, p. 5739-5744, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5739-5744.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets

Department of Anatomic Pathology, Section of Surgical Pathology,¹ Department of Clinical Pathology, Section of Clinical Microbiology,² Department of Orthopaedic Surgery, The Cleveland Clinic Foundation, Cleveland, Ohio³

Received 5 March 2004/ Returned for modification 4 May 2004/ Accepted 5 July 2004

A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus. One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus.

This article has been cited by other articles:

• Carey, B E, Nicol, L (2008). The combined oxacillin resistance and coagulase (CORC) test for rapid identification and prediction of oxacillin resistance in Staphylococcus species directly from blood culture. J. Clin. Pathol. 61: 866-868 [Abstract] [Full Text]  
• Bannoehr, J., Ben Zakour, N. L., Waller, A. S., Guardabassi, L., Thoday, K. L., van den Broek, A. H. M., Fitzgerald, J. R. (2007). Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains. J. Bacteriol. 189: 8685-8692 [Abstract] [Full Text]  
• Iwase, T., Seki, K., Shinji, H., Mizunoe, Y., Masuda, S. (2007). Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri. J Med Microbiol 56: 1346-1349 [Abstract] [Full Text]  
• Qian, Q., Eichelberger, K., Kirby, J. E. (2007). Rapid Identification of Staphylococcus aureus in Blood Cultures by Use of the Direct Tube Coagulase Test. J. Clin. Microbiol. 45: 2267-2269 [Abstract] [Full Text]  
• Chiquet, C., Pechinot, A., Creuzot-Garcher, C., Benito, Y., Croize, J., Boisset, S., Romanet, J. P., Lina, G., Vandenesch, F., for the French Institutional Endophthalmitis Study, (2007). Acute Postoperative Endophthalmitis Caused by Staphylococcus lugdunensis. J. Clin. Microbiol. 45: 1673-1678 [Abstract] [Full Text]  
• Van Hoovels, L., Vankeerberghen, A., Boel, A., Van Vaerenbergh, K., De Beenhouwer, H. (2006). First Case of Staphylococcus pseudintermedius Infection in a Human. J. Clin. Microbiol. 44: 4609-4612 [Abstract] [Full Text]  
• Bauer, T. W., Parvizi, J., Kobayashi, N., Krebs, V. (2006). Diagnosis of Periprosthetic Infection. JBJS 88: 869-882 [Abstract] [Full Text]  
• Shrestha, N. K., Tuohy, M. J., Padmanabhan, R. A., Hall, G. S., Procop, G. W. (2005). Evaluation of the LightCycler Staphylococcus MGRADE Kits on Positive Blood Cultures That Contained Gram-Positive Cocci in Clusters. J. Clin. Microbiol. 43: 6144-6146 [Abstract] [Full Text]