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Journal of Clinical Microbiology, December 2004, p. 5866-5870, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5866-5870.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Discrimination between Human Immunodeficiency Virus Type 2 Groups A and B by Real-Time PCR

Jean-Christophe Plantier,1* Marie Gueudin,1 Fabienne de Oliveira,1 Florence Damond,2 Véronique Lemée,1 Françoise Brun-Vézinet,2 and François Simon1,3

Unité de Virologie, laboratoire associé au Centre National de Référence du VIH, Equipe d'Accueil 2656, CHU Charles Nicolle, Rouen,1 Laboratoire de Virologie, INSERM U552, Hôpital Bichat, Paris, France,2 Institut Pasteur, Dakar, Sénégal3

Received 5 June 2004/ Returned for modification 6 July 2004/ Accepted 2 August 2004

We studied the feasibility of genotyping human immunodeficiency virus (HIV) type 2 groups A and B by real-time PCR. Two group-specific PCRs were developed. Real-time genotyping of 22 samples of genotype A, 10 samples of genotype B, and the isolate of new group H were compared to genotyping by sequencing and phylogeny. The group-specific PCRs specifically identified 84.3% of group A or B samples; isolate H was not detected. This method allowed rapid and specific discrimination between HIV-2 groups A and B and could be a useful tool for molecular epidemiological studies.

* Corresponding author. Mailing address: Unité de Virologie, CHU Charles Nicolle, 1 rue de Germont, 76031 Rouen, France. Phone: 33 2 32 88 82 36. Fax: 33 2 32 88 68 52. E-mail: Jean-Christophe.Plantier{at}univ-rouen.fr.

Journal of Clinical Microbiology, December 2004, p. 5866-5870, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5866-5870.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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