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Journal of Clinical Microbiology, December 2004, p. 5875-5876, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5875-5876.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Decontamination Methods and Growth Media for Primary Isolation of Mycobacterium ulcerans from Surgical Specimens

Dorothy Yeboah-Manu,1,2 Thomas Bodmer,3 Ernestina Mensah-Quainoo,4 Samuel Owusu,1 David Ofori-Adjei,1 and Gerd Pluschke2*

Noguchi Memorial Institute for Medical Research, Legon,1 Ga District Health Services, Amasaman, Ghana,4 Institute for Infectious Diseases, University of Bern, Bern,3 Swiss Tropical Institute, Basel, Switzerland2

Received 30 June 2004/ Returned for modification 21 July 2004/ Accepted 20 August 2004

We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results.

* Corresponding author. Mailing address: Swiss Tropical Institute, Socinstrasse 57, CH 4002 Basel, Switzerland. Phone: 41 61 2848235. Fax: 41 61 2718654. E-mail: Gerd.Pluschke{at}unibas.ch.

Journal of Clinical Microbiology, December 2004, p. 5875-5876, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5875-5876.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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