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Journal of Clinical Microbiology, December 2004, p. 5892-5894, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5892-5894.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Species-Directed 16S rRNA Gene PCR Primers for Detection of Atopobium vaginae in Patients with Bacterial Vaginosis

Michael J. Ferris,1,2* Alicia Masztal,2 and David H. Martin3

Department of Pediatrics,1 Department of Internal Medicine, Louisiana State University Health Sciences Center,3 The Research Institute for Children, New Orleans, Louisiana2

Received 24 May 2004/ Returned for modification 21 June 2004/ Accepted 23 August 2004

Recent studies suggest that the association between a metronidazole-resistant anaerobe, Atopobium vaginae, and bacterial vaginosis (BV) warrants further investigation. In the present study, specific primers enhanced detection of A. vaginae and provided additional evidence that this bacterium is prevalent among patients with BV but absent among patients with normal vaginal flora.

* Corresponding author. Mailing address: Research and Education Bldg., Children's Hospital, 200 Henry Clay Ave., New Orleans, LA 70118. Phone: (504) 896-2736. Fax: (504) 894-5379. E-mail: mferris{at}chnola-research.org.

Journal of Clinical Microbiology, December 2004, p. 5892-5894, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5892-5894.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.