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Journal of Clinical Microbiology, February 2004, p. 627-630, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.627-630.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Enzyme-Linked Immunosorbent Assay for Helicobacter pylori Needs Adjustment for the Population Investigated

Thi Thu Ha Hoang,1,2 Thuc-Uyen Wheeldon,3 Carina Bengtsson,1 Dac Cam Phung,2 Mikael Sörberg,3 and Marta Granström1*

Department of Clinical Microbiology, Microbiology and Tumorbiology Center,1 Division of Infectious Diseases, Department of Medicine, Karolinska Hospital, Karolinska Institutet, Stockholm, Sweden,3 National Institute of Hygiene and Epidemiology, Hanoi, Vietnam2

Received 7 May 2003/ Returned for modification 13 August 2003/ Accepted 13 November 2003

Helicobacter pylori infection and peptic ulcer disease are common in developing countries, e.g., Vietnam. An enzyme-linked immunosorbent assay (ELISA) for screening of patients and for seroepidemiology is a useful tool but needs to be validated in the population studied. We used in-house ELISA with sonicated Swedish and Vietnamese strains as antigens to measure immunoglobulin G antibodies after absorption with sonicated Campylobacter jejuni in sera from 270 H. pylori culture-confirmed peptic ulcer patients, 128 Swedish urea-breath test and immunoblot-positive healthy controls, and 432 Vietnamese immunoblot-positive population controls. Sonicated whole-cell antigen based on the local strains showed a significantly better performance. Immunoblot-positive peptic ulcer patients had significantly higher antibody concentrations than immunoblot-positive population controls, necessitating a lower cutoff level if serology is used for screening or epidemiological purposes. The study shows that the parameters of ELISA for H. pylori need to be adjusted for the population being investigated.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Karolinska Hospital, S-171 76 Stockholm, Sweden. Phone: 46-8-517 73564. Fax: 46-8-30 80 99. E-mail: marta.granstrom{at}labmed.ki.se.

Journal of Clinical Microbiology, February 2004, p. 627-630, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.627-630.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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