This Article Full Text Full Text (PDF) An erratum has been published Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Banu, S. Articles by Brosch, R. Search for Related Content PubMed PubMed Citation Articles by Banu, S. Articles by Brosch, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2004, p. 674-682, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.674-682.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotypic Analysis of Mycobacterium tuberculosis in Bangladesh and Prevalence of the Beijing Strain

ICDDR,B: Centre for Health and Population Research, Dhaka-1000,¹ Microbiology Department, Chittagong Medical College, Chittagong,³ Bangladesh; Veterinary Laboratories Agency, New Haw, Addlestone KT15 3NB, United Kingdom,² Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 75724 Paris Cedex 15, France⁴

Received 12 June 2003/ Returned for modification 6 August 2003/ Accepted 22 September 2003

Genotypic analysis was performed on 48 Mycobacterium tuberculosis complex strains collected from a hospital in Dhaka city. Deletion analysis showed that the isolates were all M. tuberculosis; 13 of them were found to be of the "ancestral" type, while 35 were of the "modern" type, indicating that both endemic (ancestral type) and epidemic (modern type) strains cause tuberculosis in Bangladesh. Genotyping based on the spoligotype and variable-number tandem repeats (VNTR) of mycobacterial interspersed repetitive units (MIRU) was also done. A total of 34 strains (71%) were grouped by spoligotyping into nine different clusters; the largest comprised 15 isolates of the Beijing genotype, whereas the remaining eight clusters consisted of two to five isolates. MIRU-VNTR typing detected 32 different patterns among 44 tested strains, and the 15 Beijing strains were further discriminated by MIRU-VNTR typing (7 distinct patterns for the 15 isolates). These results indicate that MIRU-VNTR typing, along with spoligotyping and deletion analysis, can be used effectively for molecular epidemiological studies to determine ongoing transmission clusters; to our knowledge, this is the first report about the type of strains prevailing in Bangladesh.

This article has been cited by other articles:

• Ashworth, M., Horan, K. L., Freeman, R., Oren, E., Narita, M., Cangelosi, G. A. (2008). Use of PCR-Based Mycobacterium tuberculosis Genotyping To Prioritize Tuberculosis Outbreak Control Activities. J. Clin. Microbiol. 46: 856-862 [Abstract] [Full Text]  
• Jiao, W. W., Mokrousov, I., Sun, G. Z., Guo, Y. J., Vyazovaya, A., Narvskaya, O., Shen, A D. (2008). Evaluation of New Variable-Number Tandem-Repeat Systems for Typing Mycobacterium tuberculosis with Beijing Genotype Isolates from Beijing, China. J. Clin. Microbiol. 46: 1045-1049 [Abstract] [Full Text]  
• Rahim, Z., Zaman, K., van der Zanden, A. G. M., Mollers, M. J., van Soolingen, D., Raqib, R., Zaman, K., Begum, V., Rigouts, L., Portaels, F., Rastogi, N., Sola, C. (2007). Assessment of Population Structure and Major Circulating Phylogeographical Clades of Mycobacterium tuberculosis Complex in Bangladesh Suggests a High Prevalence of a Specific Subclade of Ancient M. tuberculosis Genotypes. J. Clin. Microbiol. 45: 3791-3794 [Abstract] [Full Text]  
• Hanekom, M., van der Spuy, G. D., van Pittius, N. C. G., McEvoy, C. R. E., Ndabambi, S. L., Victor, T. C., Hoal, E. G., van Helden, P. D., Warren, R. M. (2007). Evidence that the Spread of Mycobacterium tuberculosis Strains with the Beijing Genotype Is Human Population Dependent. J. Clin. Microbiol. 45: 2263-2266 [Abstract] [Full Text]  
• Hasan, Z., Tanveer, M., Kanji, A., Hasan, Q., Ghebremichael, S., Hasan, R. (2006). Spoligotyping of Mycobacterium tuberculosis Isolates from Pakistan Reveals Predominance of Central Asian Strain 1 and Beijing Isolates.. J. Clin. Microbiol. 44: 1763-1768 [Abstract] [Full Text]  
• Mokrousov, I., Rao, K. R., Ahmed, N., Srinivas, S., Sechi, L. A., Hasnain, S. E. (2006). Mycobacterium tuberculosis Beijing Genotype and Mycobacterial Interspersed Repetitive Unit Typing. J. Clin. Microbiol. 44: 1614-1615 [Full Text]  
• Prodinger, W. M., Brandstatter, A., Naumann, L., Pacciarini, M., Kubica, T., Boschiroli, M. L., Aranaz, A., Nagy, G., Cvetnic, Z., Ocepek, M., Skrypnyk, A., Erler, W., Niemann, S., Pavlik, I., Moser, I. (2005). Characterization of Mycobacterium caprae Isolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping. J. Clin. Microbiol. 43: 4984-4992 [Abstract] [Full Text]  
• Mokrousov, I., Ly, H. M., Otten, T., Lan, N. N., Vyshnevskyi, B., Hoffner, S., Narvskaya, O. (2005). Origin and primary dispersal of the Mycobacterium tuberculosis Beijing genotype: Clues from human phylogeography. Genome Res 15: 1357-1364 [Abstract] [Full Text]  
• Jou, R., Chiang, C.-Y., Huang, W.-L. (2005). Distribution of the Beijing Family Genotypes of Mycobacterium tuberculosis in Taiwan. J. Clin. Microbiol. 43: 95-100 [Abstract] [Full Text]  
• Aranaz, A., Romero, B., Montero, N., Alvarez, J., Bezos, J., de Juan, L., Mateos, A., Dominguez, L. (2004). Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain. J. Clin. Microbiol. 42: 5388-5391 [Abstract] [Full Text]