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Journal of Clinical Microbiology, February 2004, p. 683-692, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.683-692.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of TaqMan 5' Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic

Jørgen Skov Jensen,1* Eva Björnelius,2 Birthe Dohn,1 and Peter Lidbrink2

Mycoplasma Laboratory, Statens Serum Institut, DK-2300 Copenhagen S, Denmark,1 Department of Dermatovenereology, Huddinge University Hospital, Karolinska Institutet, S-14 186 Huddinge, Sweden2

Received 4 May 2003/ Returned for modification 27 June 2003/ Accepted 29 October 2003

Mycoplasma genitalium is a cause of nongonococcal urethritis, particularly in patients not infected with Chlamydia trachomatis. A quantitative 5' nuclease assay (TaqMan PCR) was developed and validated. The assay detected a fragment of the MgPa adhesin gene by use of a TaqMan MGB (minor groove binder) probe and included an internal processing control to detect PCR inhibition. Urethral swab specimens and first-void urine samples from M. genitalium-positive men were examined, and the M. genitalium DNA load was correlated to symptoms and signs. The assay consistently detected <5 genome copies without cross-reactions with other mycoplasmas. Urine and urethral swab specimens from men with urethritis had higher M. genitalium DNA loads than specimens from men without urethritis. However, a very broad overlap of DNA loads between patients with and without urethritis was observed. Urethral swab specimens from patients with urethral discharge had a significantly higher DNA load than specimens from patients without discharge. This correlation was not found in first-void urine specimens.

* Corresponding author. Mailing address: Mycoplasma Laboratory, Statens Seruminstitut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 3268 3636. Fax: 45 3268 3152. E-mail: jsj{at}ssi.dk.

Journal of Clinical Microbiology, February 2004, p. 683-692, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.683-692.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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