This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jensen, J. S.
Right arrow Articles by Lidbrink, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jensen, J. S.
Right arrow Articles by Lidbrink, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2004, p. 683-692, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.683-692.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of TaqMan 5' Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic

Jørgen Skov Jensen,1* Eva Björnelius,2 Birthe Dohn,1 and Peter Lidbrink2

Mycoplasma Laboratory, Statens Serum Institut, DK-2300 Copenhagen S, Denmark,1 Department of Dermatovenereology, Huddinge University Hospital, Karolinska Institutet, S-14 186 Huddinge, Sweden2

Received 4 May 2003/ Returned for modification 27 June 2003/ Accepted 29 October 2003

Mycoplasma genitalium is a cause of nongonococcal urethritis, particularly in patients not infected with Chlamydia trachomatis. A quantitative 5' nuclease assay (TaqMan PCR) was developed and validated. The assay detected a fragment of the MgPa adhesin gene by use of a TaqMan MGB (minor groove binder) probe and included an internal processing control to detect PCR inhibition. Urethral swab specimens and first-void urine samples from M. genitalium-positive men were examined, and the M. genitalium DNA load was correlated to symptoms and signs. The assay consistently detected <5 genome copies without cross-reactions with other mycoplasmas. Urine and urethral swab specimens from men with urethritis had higher M. genitalium DNA loads than specimens from men without urethritis. However, a very broad overlap of DNA loads between patients with and without urethritis was observed. Urethral swab specimens from patients with urethral discharge had a significantly higher DNA load than specimens from patients without discharge. This correlation was not found in first-void urine specimens.

* Corresponding author. Mailing address: Mycoplasma Laboratory, Statens Seruminstitut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 3268 3636. Fax: 45 3268 3152. E-mail: jsj{at}ssi.dk.

Journal of Clinical Microbiology, February 2004, p. 683-692, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.683-692.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bradshaw, C S, Fairley, C K, Lister, N A, Chen, S J, Garland, S M, Tabrizi, S N (2009). Mycoplasma genitalium in men who have sex with men at male-only saunas. Sex. Transm. Infect. 85: 432-435 [Abstract] [Full Text]  
  • Shahmanesh, M, Moi, H, Lassau, F, Janier, M (2009). 2009 European Guideline on the Management of Male Non-gonococcal Urethritis. Int J STD AIDS 20: 458-464 [Full Text]  
  • Chalker, V. J., Jordan, K., Ali, T., Ison, C. (2009). Real-time PCR detection of the mg219 gene of unknown function of Mycoplasma genitalium in men with and without non-gonococcal urethritis and their female partners in England. J Med Microbiol 58: 895-899 [Abstract] [Full Text]  
  • Mckechnie, M. L., Hillman, R., Couldwell, D., Kong, F., Freedman, E., Wang, H., Gilbert, G. L. (2009). Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR-Based Reverse Line Blot Assay. J. Clin. Microbiol. 47: 1871-1877 [Abstract] [Full Text]  
  • Musatovova, O., Baseman, J. B. (2009). Analysis Identifying Common and Distinct Sequences among Texas Clinical Strains of Mycoplasma genitalium. J. Clin. Microbiol. 47: 1469-1475 [Abstract] [Full Text]  
  • Moi, H, Reinton, N, Moghaddam, A (2009). Mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men. Sex. Transm. Infect. 85: 15-18 [Abstract] [Full Text]  
  • Moi, H, Reinton, N, Moghaddam, A (2009). Mycoplasma genitalium in women with lower genital tract inflammation. Sex. Transm. Infect. 85: 10-14 [Abstract] [Full Text]  
  • Edberg, A., Aronsson, F., Johansson, E., Wikander, E., Ahlqvist, T., Fredlund, H. (2009). Endocervical swabs transported in first void urine as combined specimens in the detection of Mycoplasma genitalium by real-time PCR. J Med Microbiol 58: 117-120 [Abstract] [Full Text]  
  • Westh, H, Jensen, J S (2008). Low prevalence of the new variant of Chlamydia trachomatis in Denmark. Sex. Transm. Infect. 84: 546-547 [Abstract] [Full Text]  
  • Jernberg, E, Moghaddam, A, Moi, H (2008). Azithromycin and moxifloxacin for microbiological cure of Mycoplasma genitalium infection: an open study. Int J STD AIDS 19: 676-679 [Abstract] [Full Text]  
  • Shimizu, T., Kida, Y., Kuwano, K. (2008). A Triacylated Lipoprotein from Mycoplasma genitalium Activates NF-{kappa}B through Toll-Like Receptor 1 (TLR1) and TLR2. Infect. Immun. 76: 3672-3678 [Abstract] [Full Text]  
  • Hamasuna, R, Imai, H, Tsukino, H, Jensen, J S, Osada, Y (2008). Prevalence of Mycoplasma genitalium among female students in vocational schools in Japan. Sex. Transm. Infect. 84: 303-305 [Abstract] [Full Text]  
  • Edberg, A., Jurstrand, M., Johansson, E., Wikander, E., Hoog, A., Ahlqvist, T., Falk, L., Jensen, J. S., Fredlund, H. (2008). A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and women. J Med Microbiol 57: 304-309 [Abstract] [Full Text]  
  • Magbanua, J. P. V, Goh, B. T., Michel, C.-E., Aguirre-Andreasen, A., Alexander, S., Ushiro-Lumb, I., Ison, C., Lee, H. (2007). Chlamydia trachomatis variant not detected by plasmid based nucleic acid amplification tests: molecular characterisation and failure of single dose azithromycin. Sex. Transm. Infect. 83: 339-343 [Abstract] [Full Text]  
  • Baczynska, A., Funch, P., Fedder, J., Knudsen, H. J., Birkelund, S., Christiansen, G. (2007). Morphology of human Fallopian tubes after infection with Mycoplasma genitalium and Mycoplasma hominis--in vitro organ culture study. Hum Reprod 22: 968-979 [Abstract] [Full Text]  
  • Hamasuna, R., Osada, Y., Jensen, J. S. (2007). Isolation of Mycoplasma genitalium from First-Void Urine Specimens by Coculture with Vero Cells. J. Clin. Microbiol. 45: 847-850 [Abstract] [Full Text]  
  • Wroblewski, J. K. H., Manhart, L. E., Dickey, K. A., Hudspeth, M. K., Totten, P. A. (2006). Comparison of Transcription-Mediated Amplification and PCR Assay Results for Various Genital Specimen Types for Detection of Mycoplasma genitalium.. J. Clin. Microbiol. 44: 3306-3312 [Abstract] [Full Text]  
  • Svenstrup, H. F., Jensen, J. S., Gevaert, K., Birkelund, S., Christiansen, G. (2006). Identification and Characterization of Immunogenic Proteins of Mycoplasma genitalium.. CVI 13: 913-922 [Abstract] [Full Text]  
  • Ross, J D C, Jensen, J S (2006). Mycoplasma genitalium as a sexually transmitted infection: implications for screening, testing, and treatment.. Sex. Transm. Infect. 82: 269-271 [Abstract] [Full Text]  
  • Iverson-Cabral, S. L., Astete, S. G., Cohen, C. R., Rocha, E. P. C., Totten, P. A. (2006). Intrastrain Heterogeneity of the mgpB Gene in Mycoplasma genitalium Is Extensive In Vitro and In Vivo and Suggests that Variation Is Generated via Recombination with Repetitive Chromosomal Sequences. Infect. Immun. 74: 3715-3726 [Abstract] [Full Text]  
  • Hjorth, S. V., Bjornelius, E., Lidbrink, P., Falk, L., Dohn, B., Berthelsen, L., Ma, L., Martin, D. H., Jensen, J. S. (2006). Sequence-Based Typing of Mycoplasma genitalium Reveals Sexual Transmission.. J. Clin. Microbiol. 44: 2078-2083 [Abstract] [Full Text]  
  • Hardick, J., Giles, J., Hardick, A., Hsieh, Y.-H., Quinn, T., Gaydos, C. (2006). Performance of the Gen-Probe Transmission-Mediated Amplification Research Assay Compared to That of a Multitarget Real-Time PCR for Mycoplasma genitalium Detection. J. Clin. Microbiol. 44: 1236-1240 [Abstract] [Full Text]  
  • Musatovova, O., Herrera, C., Baseman, J. B. (2006). Proximal Region of the Gene Encoding Cytadherence-Related Protein Permits Molecular Typing of Mycoplasma genitalium Clinical Strains by PCR-Restriction Fragment Length Polymorphism. J. Clin. Microbiol. 44: 598-603 [Abstract] [Full Text]  
  • Hamasuna, R., Osada, Y., Jensen, J. S. (2005). Antibiotic Susceptibility Testing of Mycoplasma genitalium by TaqMan 5' Nuclease Real-Time PCR. Antimicrob. Agents Chemother. 49: 4993-4998 [Abstract] [Full Text]  
  • Anagrius, C, Lore, B, Jensen, J S (2005). Mycoplasma genitalium: prevalence, clinical significance, and transmission. Sex. Transm. Infect. 81: 458-462 [Abstract] [Full Text]  
  • Svenstrup, H. F., Jensen, J. S., Bjornelius, E., Lidbrink, P., Birkelund, S., Christiansen, G. (2005). Development of a Quantitative Real-Time PCR Assay for Detection of Mycoplasma genitalium. J. Clin. Microbiol. 43: 3121-3128 [Abstract] [Full Text]  
  • Jurstrand, M., Jensen, J. S., Fredlund, H., Falk, L., Molling, P. (2005). Detection of Mycoplasma genitalium in urogenital specimens by real-time PCR and by conventional PCR assay. J Med Microbiol 54: 23-29 [Abstract] [Full Text]  
  • Wada, T., Maeda, S., Tamaru, A., Imai, S., Hase, A., Kobayashi, K. (2004). Dual-Probe Assay for Rapid Detection of Drug-Resistant Mycobacterium tuberculosis by Real-Time PCR. J. Clin. Microbiol. 42: 5277-5285 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»