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Journal of Clinical Microbiology, February 2004, p. 718-721, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.718-721.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multicenter Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with the NCCLS M27-A2 Reference Method for Testing New Antifungal Agents against Clinical Isolates of Candida spp.

A. Espinel-Ingroff,1* M. Pfaller,2 S. A. Messer,2 C. C. Knapp,3 N. Holliday,3 and S. B. Killian3

VCU Medical Center, Richmond, Virginia,1 University of Iowa College of Medicine, Iowa City, Iowa ,2 TREK Diagnostic Systems, Cleveland, Ohio3

Received 29 August 2003/ Returned for modification 14 October 2003/ Accepted 24 October 2003

A multicenter (three centers) study compared MICs obtained by the Sensititre YeastOne Colorimetric Antifungal plate to reference microdilution broth (NCCLS M27-A2 document) MICs of three new triazoles (posaconazole, ravuconazole, and voriconazole) and the echinocandin caspofungin acetate for 100 isolates of Candida spp. In addition, amphotericin B and fluconazole were tested as control drugs. Colorimetric MICs of caspofungin and amphotericin B corresponded to the first blue well (no growth), and MICs of the other agents corresponded to the first slightly purple or blue well. Two comparisons of MIC pairs by the two methods were evaluated: 24-h colorimetric MICs were compared to NCCLS MICs at 24 and at 48 h. The interlaboratory reproducibility of YeastOne and reference MICs was also examined. The best performance of the YeastOne plate was with 24-h MICs (overall, 95 to 99% agreement) for all the species and antifungal agents. These results suggest the potential value of the YeastOne plate for use in the clinical laboratory for the four new antifungal agents evaluated.

* Corresponding author. Mailing Address: Medical College of Virginia of Virginia Commonwealth University, P. O. Box 980049, Richmond, VA 23298-0049. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail: avingroff{at}hsc.vcu.edu.

Journal of Clinical Microbiology, February 2004, p. 718-721, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.718-721.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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