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Journal of Clinical Microbiology, February 2004, p. 734-740, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.734-740.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prospective Study of Use of PCR Amplification and Sequencing of 16S Ribosomal DNA from Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis in a Clinical Setting

Tim Schuurman,* Richard F. de Boer, Anna M. D. Kooistra-Smid, and Anton A. van Zwet

Department of Research and Development, Regional Public Health Laboratory for Groningen and Drenthe, Groningen, The Netherlands

Received 3 July 2003/ Returned for modification 7 September 2003/ Accepted 27 October 2003

We have evaluated the use of a broad-range PCR aimed at the 16S rRNA gene in detecting bacterial meningitis in a clinical setting. To achieve a uniform DNA extraction procedure for both gram-positive and gram-negative organisms, a combination of physical disruption (bead beating) and a silica-guanidiniumthiocyanate procedure was used for nucleic acid preparation. To diminish the risk of contamination as much as possible, we chose to amplify almost the entire 16S rRNA gene. The analytical sensitivity of the assay was approximately 1 x 102 to 2 x 102 CFU/ml of cerebrospinal fluid (CSF) for both gram-negative and gram-positive bacteria. In a prospective study of 227 CSF samples, broad-range PCR proved to be superior to conventional methods in detecting bacterial meningitis when antimicrobial therapy had already started. Overall, our assay showed a sensitivity of 86%, a specificity of 97%, a positive predictive value of 80%, and a negative predictive value of 98% compared to culture. We are currently adapting the standard procedures in our laboratory for detecting bacterial meningitis; broad-range 16S ribosomal DNA PCR detection is indicated when antimicrobial therapy has already started at time of lumbar puncture or when cultures remain negative, although the suspicion of bacterial meningitis remains.

* Corresponding author. Mailing address: Department of Research and Development, Regional Public Health Laboratory for Groningen and Drenthe, Van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands. Phone: 31-50-5215247. Fax: 31-50-5271488. E-mail: t.schuurman{at}slgd.nl.

Journal of Clinical Microbiology, February 2004, p. 734-740, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.734-740.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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