This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsuoka, M.
Right arrow Articles by Izumi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsuoka, M.
Right arrow Articles by Izumi, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2004, p. 741-745, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.741-745.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotyping of Mycobacterium leprae on the Basis of the Polymorphism of TTC Repeats for Analysis of Leprosy Transmission

Masanori Matsuoka,1* Liangfen Zhang,1 Teky Budiawan,2 Keisuke Saeki,3 and Shinzo Izumi4

Leprosy Research Center, National Institute of Infectious Diseases, Tokyo,1 National Leprosarium Oshima-Seisho-En, Kagawa, Japan,3 and Leprosy-TB Program, Provincial Health Service, Manado, North Sulawesi,2 Leprosy Study Group, Tropical Disease Center, Kampus C UNAIR, Airlangga University, Surabaya, Indonesia4

Received 4 July 2003/ Returned for modification 20 August 2003/ Accepted 3 November 2003

The polymorphism of TTC repeats in Mycobacterium leprae was examined using the bacilli obtained from residents in villages at North Maluku where M. leprae infections are highly endemic (as well as from patients at North Sulawesi of Indonesia) to elucidate the possible mode of leprosy transmission. TTC genotypes are stable for several generations of passages in nude mice footpads and, hence, are feasible for the genotyping of isolates and epidemiological analysis of leprosy transmission. It was found that bacilli with different TTC genotypes were distributed among residents at the same dwelling in villages in which leprosy is endemic and that some household contacts harbored bacilli with a different genotype from that harbored by the patient. Investigations of a father-and-son pair of patients indicated that infections of bacilli with 10 and 18 copies, respectively, had occurred. Genotypes of TTC repeats were found to differ between a son under treatment and two brothers. These results reveal the possibility that in addition to exposure via the presence of a leprosy patient with a multibacillary infection who was living with family members, there might have been some infectious sources to which the residents had been commonly exposed outside the dwellings. A limited discriminative capacity of the TTC polymorphism in the epidemiological analysis implies the need of searching other useful polymorphic loci for detailed subdivision of clinical isolates.

* Corresponding author. Mailing address: Leprosy Research Center, National Institute of Infectious Diseases, 4-2-1, Aobacho, Higashimurayama-shi, Tokyo, 189-0002, Japan. Phone: 81-42-391-8211. Fax: 81-42-394-9092. E-mail: matsuoka{at}nih.go.jp.

Journal of Clinical Microbiology, February 2004, p. 741-745, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.741-745.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kimura, M., Sakamuri, R. M., Groathouse, N. A., Rivoire, B. L., Gingrich, D., Krueger-Koplin, S., Cho, S.-N., Brennan, P. J., Vissa, V. (2009). Rapid Variable-Number Tandem-Repeat Genotyping for Mycobacterium leprae Clinical Specimens. J. Clin. Microbiol. 47: 1757-1766 [Abstract] [Full Text]  
  • Weng, X., Wang, Z., Liu, J., Kimura, M., Black, W. C. IV, Brennan, P. J., Li, H., Vissa, V. D. (2007). Identification and Distribution of Mycobacterium leprae Genotypes in a Region of High Leprosy Prevalence in China: a 3-Year Molecular Epidemiological Study. J. Clin. Microbiol. 45: 1728-1734 [Abstract] [Full Text]  
  • Groathouse, N. A., Brown, S. E., Knudson, D. L., Brennan, P. J., Slayden, R. A. (2006). Isothermal Amplification and Molecular Typing of the Obligate Intracellular Pathogen Mycobacterium leprae Isolated from Tissues of Unknown Origins. J. Clin. Microbiol. 44: 1502-1508 [Abstract] [Full Text]  
  • Zhang, L., Budiawan, T., Matsuoka, M. (2005). Diversity of Potential Short Tandem Repeats in Mycobacterium leprae and Application for Molecular Typing. J. Clin. Microbiol. 43: 5221-5229 [Abstract] [Full Text]  
  • Young, S. K., Taylor, G. M., Jain, S., Suneetha, L. M., Suneetha, S., Lockwood, D. N. J., Young, D. B. (2004). Microsatellite Mapping of Mycobacterium leprae Populations in Infected Humans. J. Clin. Microbiol. 42: 4931-4936 [Abstract] [Full Text]  
  • Truman, R., Fontes, A. B., de Miranda, A. B., Suffys, P., Gillis, T. (2004). Genotypic Variation and Stability of Four Variable-Number Tandem Repeats and Their Suitability for Discriminating Strains of Mycobacterium leprae. J. Clin. Microbiol. 42: 2558-2565 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»