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Journal of Clinical Microbiology, February 2004, p. 835-836, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.835-836.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Primer Pair p289-p290, Designed To Detect Both Noroviruses and Sapoviruses by Reverse Transcription-PCR, Also Detects Rotaviruses by Cross-Reactivity

Juan E. Ludert,* Ana C. Alcalá, and Ferdinando Liprandi

Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela

Received 23 October 2003/ Accepted 31 October 2003

A primer pair (p289-p290) designed to detect both noroviruses and sapoviruses by reverse transcription-PCR (Jiang et al., J. Virol. Methods 83:145, 1999) cross-reacts with rotaviruses. The rotavirus amplicon corresponds to genome segment 1. Furthermore, primer pair p289-p290 detected rotaviruses as efficiently as rotavirus-specific primers directed to rotavirus gene 4.

* Corresponding author. Mailing address: Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas 1020-A, Venezuela. Phone: 58-212-5041655. Fax: 58-212-5041382. E-mail: jeludert{at}ivic.ve.

Journal of Clinical Microbiology, February 2004, p. 835-836, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.835-836.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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