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Journal of Clinical Microbiology, February 2004, p. 844-846, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.844-846.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Serum-Based Taqman Real-Time PCR Assay for Diagnosis of Invasive Aspergillosis

Svetlana Challier,* Stéphanie Boyer, Eric Abachin, and Patrick Berche

Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris, France

Received 28 July 2003/ Returned for modification 31 August 2003/ Accepted 27 October 2003

We compared an Aspergillus fumigatus-specific Taqman real-time PCR assay for the diagnosis of invasive aspergillosis (IA) with the enzyme-linked immunosorbent assay Platelia Aspergillus method. Patients with proven or probable IA had positive results with at least one of the two methods. The PCR method seems to be more specific, and a combination of the two should improve the diagnosis of IA.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, 149-161 rue de Sèvres, 75743 Paris cedex 15, France. Phone: 33-1-44-49-49-61. Fax: 33-1-44-49-49-60. E-mail: svetlana.challier{at}nck.ap-hop-paris.fr.

Journal of Clinical Microbiology, February 2004, p. 844-846, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.844-846.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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