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Journal of Clinical Microbiology, February 2004, p. 906-908, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.906-908.2004
Copyright © 2004, American Society of Microbiology. All Rights Reserved.

Novel Method Using a Laser Scanning Cytometer for Detection of Mycobacteria in Clinical Samples

Cidália Pina-Vaz,1,2,3* Sofia Costa-Oliveira,1,2 Acácio Gonçalves Rodrigues,1,2 and Alexandre Salvador2

Department of Microbiology, Faculty of Medicine,1 IPATIMUP—Institute of Pathology and Molecular Immunology, University of Porto,2 Department of Microbiology, Hospital S. João, Porto, Portugal3

Received 22 May 2003/ Returned for modification 19 August 2003/ Accepted 28 August 2003

In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Medicine, University of Porto, Alameda Prof. Hernani Monteiro, 4200 Porto, Portugal. Phone: 351 91 919358514. Fax: 351-22-9962096. E-mail: micteam{at}clix.pt.

Journal of Clinical Microbiology, February 2004, p. 906-908, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.906-908.2004
Copyright © 2004, American Society of Microbiology. All Rights Reserved.



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