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Journal of Clinical Microbiology, March 2004, p. 1069-1074, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1069-1074.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Recombinant Fragments of Entamoeba histolytica Gal/GalNAc Lectin Intermediate Subunit for Serodiagnosis of Amebiasis

Hiroshi Tachibana,^1* Xun-Jia Cheng,^1,2 Gohta Masuda,^3, Noriyuki Horiki,^4,5 and Tsutomu Takeuchi⁶

Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa,¹ Tokyo Metropolitan Komagome Hospital, Bunkyo-ku,³ Division of Internal Medicine, St. Luke's International Hospital, Chuo-ku,⁵ Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku-ku, Tokyo,⁶ Third Department of Internal Medicine, Mie University School of Medicine, Tsu, Mie, Japan,⁴ Department of Medical Microbiology and Parasitology, Fudan University School of Medicine, Shanghai, China²

Received 18 September 2003/ Returned for modification 10 November 2003/ Accepted 24 November 2003

We have recently identified a 150-kDa surface antigen of Entamoeba histolytica as an intermediate subunit (Igl) of galactose- and N-acetyl-D-galactosamine-inhibitable lectin, which is a cysteine-rich protein consisting of 1,101 amino acids (aa) and containing multiple CXXC motifs in amino acid sequences. In the present study, full-length Igl except for the signal sequences (aa 14 to 1088) and three fragments of Igl—the N-terminal part (aa 14 to 382), the middle part (aa 294 to 753), and the C-terminal part (aa 603 to 1088)—were prepared in Escherichia coli, and the reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunosorbent assay (ELISA). Sera from 57 symptomatic patients with amebic liver abscess or amebic colitis, sera from 15 asymptomatic cyst passers, sera from 40 individuals with other protozoan infections, and sera from 50 healthy controls were used. The sensitivity and specificity of the recombinant full-length Igl in the ELISA were 90 and 94%, respectively. When three fragments were used as antigens in the ELISA, the sensitivities were 56% in the N terminus, 92% in the middle part, and 97% in the C terminus. The specificities of the three antigens were 96% in the N terminus and 99% in both the middle and C-terminal fragments. These results demonstrate that Igl is well recognized in not only symptomatic but also asymptomatic patients with E. histolytica infection and that the carboxyl terminus of Igl is an especially useful antigen for the serodiagnosis of amebiasis.

Present address: Tokyo Metropolitan Kita Medical and Rehabilitation Center, Kita-ku, Tokyo, Japan.

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