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Journal of Clinical Microbiology, March 2004, p. 1115-1122, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1115-1122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis

M. Baele,1* K. Van den Bulck,1 A. Decostere,1 P. Vandamme,2 M.-L. Hänninen,3 R. Ducatelle,1 and F. Haesebrouck1

Department of Pathology, Bacteriology, and Avian Diseases,1 Laboratory of Microbiology, Ghent University, Merelbeke, Belgium,2 Department of Food and Environmental Hygiene, University of Helsinki, Helsinki, Finland3

Received 4 March 2003/ Returned for modification 19 August 2003/ Accepted 5 November 2003

Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis are frequently found in the gastric mucous membrane of dogs and cats. These large spiral organisms are phylogenetically highly related to each other. Their fastidious nature makes it difficult to cultivate them in vitro, hampering traditional identification methods. We describe here a multiplex PCR test based on the tRNA intergenic spacers and on the urease gene, combined with capillary electrophoresis, that allows discrimination of these three species. In combination with previously described 16S ribosomal DNA-based primers specific for the nonculturable "Candidatus Helicobacter suis," our procedure was shown to be very useful in determining the species identity of "Helicobacter heilmannii"-like organisms observed in human stomachs and will facilitate research concerning their possible zoonotic importance.

* Corresponding author. Mailing address: Department of Veterinary Bacteriology, Ghent University, Salisburylaan 133, Merelbeke, Belgium. Phone: (32) 9-264-74-34. Fax: (32) 9-264-74-94. E-mail: margo.baele{at}rug.ac.be.

Journal of Clinical Microbiology, March 2004, p. 1115-1122, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1115-1122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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