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Journal of Clinical Microbiology, March 2004, p. 1228-1235, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1228-1235.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Improved Antigenic Methods for Differential Diagnosis of Bovine, Caprine, and Cervine Alphaherpesviruses Related to Bovine Herpesvirus 1

Véronique Keuser,¹ Frédéric Schynts,^1, Bruno Detry,¹ Alfred Collard,² Béatrice Robert,² Alain Vanderplasschen,¹ Paul-Pierre Pastoret,^1, and Etienne Thiry^1*

Department of Infectious and Parasitic Diseases, Laboratory of Virology and Immunology, Faculty of Veterinary Medicine, University of Liège, B 4000 Liège,¹ Department of Animal Immunology, Centre d'Economie rurale, B 6900 Marloie, Belgium²

Received 18 July 2003/ Returned for modification 5 October 2003/ Accepted 11 November 2003

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four other alphaherpesviruses of ruminants—namely, BoHV-5, caprine herpesvirus 1 (CpHV-1), cervine herpesvirus 1 (CvHV-1) and CvHV-2—diagnostic tests able to discriminate BoHV-1 from these related viruses are needed to avoid misdiagnosis, especially because some of these viruses are able to cross the species barrier. In this study, murine monoclonal antibodies (MAbs) specific for BoHV-1, BoHV-5, CpHV-1, CvHV-1, and CvHV-2 were produced with the aim of setting up an immunofluorescence assay able to discriminate between these related herpesviruses. Produced MAbs were selected for their viral specificity by enzyme-linked immunosorbent assay and indirect immunofluorescence staining of virus-infected cells. Radioimmunoprecipitation characterization of the selected MAbs revealed that four of them are directed against glycoprotein C (gC) and one of them is directed against gD of these related viruses. The obtained results demonstrate that the antibodies produced allow an unambiguous discrimination of each of the four alphaherpesviruses related to BoHV-1.

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* Corresponding author. Mailing address: Department of Infectious and Parasitic Diseases, Virology, Faculty of Veterinary Medicine, Boulevard de Colonster 20, B43 b, B-4000 Liège, Belgium. Phone: 32 4 366 42 50. Fax: 32 4 366 42 61. E-mail: etienne.thiry{at}ulg.ac.be.

Present address: Department of Animal Virology, CER, B 6900 Marloie, Belgium.

Present address: Institute of Animal Health, Compton, Newbury, Berkshire, RG20 7NN London, Great Britain.

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Journal of Clinical Microbiology, March 2004, p. 1228-1235, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1228-1235.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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