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Journal of Clinical Microbiology, March 2004, p. 1243-1246, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1243-1246.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Preferential Presence of Decorin-Binding Protein B (BBA25) and BBA50 Antibodies in Cerebrospinal Fluid of Patients with Neurologic Lyme Disease

Erol Fikrig,1* Patricia K. Coyle,2 Steven E. Schutzer,3 Manchuan Chen,4 Zhidian Deng,2 and Richard A. Flavell4

Section of Rheumatology, Department of Internal Medicine,1 Section of Immunobiology and the Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520,4 Department of Neurology, State University of New York at Stony Brook, Stony Brook, New York 11794,2 Department of Medicine, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 071033

Received 15 July 2003/ Returned for modification 11 November 2003/ Accepted 3 December 2003

Borrelia burgdorferi antibodies preferentially present in cerebrospinal fluid (CSF) were examined by differentially probing a B. burgdorferi expression library with CSF and sera from patients with neurologic Lyme disease. Several phage clones selectively reacted with CSF, and these genes were then expressed in recombinant form and used to detect specific antibody in an enzyme-linked immunosorbent assay. Decorin-binding protein B (BBA25) and BBA50 (hypothetical protein) elicited immunoglobulin G (IgG) or IgM detectable in CSF—but not sera—of patients, demonstrating preferential antibody production during neuroborreliosis.

* Corresponding author. Mailing address: Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, Room 525A, 300 Cedar St., New Haven, CT 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: erol.fikrig{at}yale.edu.

Journal of Clinical Microbiology, March 2004, p. 1243-1246, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1243-1246.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Livengood, J. A., Schmit, V. L., Gilmore, R. D. Jr. (2008). Global Transcriptome Analysis of Borrelia burgdorferi during Association with Human Neuroglial Cells. Infect. Immun. 76: 298-307 [Abstract] [Full Text]  
  • Grab, D. J., Perides, G., Dumler, J. S., Kim, K. J., Park, J., Kim, Y. V., Nikolskaia, O., Choi, K. S., Stins, M. F., Kim, K. S. (2005). Borrelia burgdorferi, Host-Derived Proteases, and the Blood-Brain Barrier. Infect. Immun. 73: 1014-1022 [Abstract] [Full Text]  


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