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Journal of Clinical Microbiology, March 2004, p. 1277-1279, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1277-1279.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Different Methods To Detect Methicillin Resistance in Small-Colony Variants of Staphylococcus aureus

Frank Kipp, Karsten Becker, Georg Peters, and Christof von Eiff*

Institute of Medical Microbiology, University Hospital of Münster, 48149 Münster, Germany

Received 28 June 2003/ Returned for modification 10 November 2003/ Accepted 19 November 2003

To evaluate different methods for their abilities to detect methicillin resistance in small-colony variants (SCVs) of Staphylococcus aureus, 11 different methicillin-resistant S. aureus (MRSA) clones with the SCV phenotype were used in this study. The slow growth of SCVs often makes testing by disk diffusion or by automated methods invalid. Only detection of the mecA gene by PCR and the MRSA-Screen latex agglutination test using a higher colony number were shown to be reliable methods to rapidly detect methicillin resistance in these variants.

* Corresponding author. Mailing address: University of Münster, Institute of Medical Microbiology, D-48149 Münster, Germany. Phone: (49) 251 83-55360. Fax: (49) 251 83-55350. E-mail: eiffc{at}uni-muenster.de.

Journal of Clinical Microbiology, March 2004, p. 1277-1279, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1277-1279.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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