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Journal of Clinical Microbiology, March 2004, p. 1280-1282, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1280-1282.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Assessment of Evolution of Pandemic Vibrio parahaemolyticus by Multilocus Sequence Typing

Nandini Roy Chowdhury,1,2 O. Colin Stine,2* J. Glenn Morris,2 and G. B. Nair1,3

National Institute of Cholera and Enteric Diseases, Calcutta, India,1 International Centre for Diarrhoeal Diseases, Bangladesh, Dhaka, Bangladesh,3 University of Maryland School of Medicine, Baltimore, Maryland2

Received 8 April 2003/ Returned for modification 25 August 2003/ Accepted 21 November 2003

The genetic relatedness of 81 isolates of Vibrio parahaemolyticus was assessed by multilocus sequence typing. The strain with serotype O3:K6 emerged as a pandemic pathogen in 1996, with subsequent expansion to include strains having serotypes O1:KUT, O4:K68, and O1:K25. Sequence data from gyrB, recA, dnaE, and gnd revealed that 16 distinct serogroups isolated prior to the pandemic were highly variable and only isolates of serogroup O3:K6 shared two alleles with the pandemic strains. The pandemic strains regardless of serotype were clonal, with 51 of 54 isolates having the identical allelic profile (AP). Serotype alone did not adequately define a pandemic strain: among O1:KUT strains tested, seven strains with the identical pandemic AP carried previously described pandemic markers, while five nonpandemic strains had five distinct APs. Our sequence data provide strong molecular support for the clonal origin of pandemic V. parahaemolyticus O3:K6 and suggest that strains within such a clonal group may acquire previously identified serotypes.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland, Howard Hall 596, 660 W. Redwood St., Baltimore, MD 21201. Phone: (410) 706-1607. Fax: (410) 706-1644. E-mail: ostin001{at}umaryland.edu.

Journal of Clinical Microbiology, March 2004, p. 1280-1282, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1280-1282.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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