This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Probert, W. S.
Right arrow Articles by Graves, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Probert, W. S.
Right arrow Articles by Graves, M. H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2004, p. 1290-1293, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1290-1293.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Real-Time Multiplex PCR Assay for Detection of Brucella spp., B. abortus, and B. melitensis

William S. Probert,* Kimmi N. Schrader, Nhi Y. Khuong, Susan L. Bystrom, and Margot H. Graves

Microbial Diseases Laboratory, Division of Communicable Disease Control, California Department of Health Services, Richmond, California 94804

Received 4 August 2003/ Returned for modification 16 September 2003/ Accepted 1 October 2003

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

* Corresponding author. Mailing address: Microbial Diseases Laboratory, 850 Marina Bay Pkwy., Richmond, CA 94804. Phone: (510) 412-3700. Fax: (510) 412-3706. E-mail: wprobert{at}dhs.ca.gov.

Journal of Clinical Microbiology, March 2004, p. 1290-1293, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1290-1293.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tiller, R. V., De, B. K., Boshra, M., Huynh, L. Y., Van Ert, M. N., Wagner, D. M., Klena, J., Mohsen, T. S., El-Shafie, S. S., Keim, P., Hoffmaster, A. R., Wilkins, P. P., Pimentel, G. (2009). Comparison of Two Multiple-Locus Variable-Number Tandem-Repeat Analysis Methods for Molecular Strain Typing of Human Brucella melitensis Isolates from the Middle East. J. Clin. Microbiol. 47: 2226-2231 [Abstract] [Full Text]  
  • Foster, J. T., Okinaka, R. T., Svensson, R., Shaw, K., De, B. K., Robison, R. A., Probert, W. S., Kenefic, L. J., Brown, W. D., Keim, P. (2008). Real-Time PCR Assays of Single-Nucleotide Polymorphisms Defining the Major Brucella Clades. J. Clin. Microbiol. 46: 296-301 [Abstract] [Full Text]  
  • Mukherjee, F., Jain, J., Patel, V., Nair, M. (2007). Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals. J Med Microbiol 56: 1309-1316 [Abstract] [Full Text]  
  • Pingle, M. R., Granger, K., Feinberg, P., Shatsky, R., Sterling, B., Rundell, M., Spitzer, E., Larone, D., Golightly, L., Barany, F. (2007). Multiplexed Identification of Blood-Borne Bacterial Pathogens by Use of a Novel 16S rRNA Gene PCR-Ligase Detection Reaction-Capillary Electrophoresis Assay. J. Clin. Microbiol. 45: 1927-1935 [Abstract] [Full Text]  
  • Mutnal, M. B., Purwar, S., Metgud, S. C., Nagmoti, M. B., Patil, C. S. (2007). PCR confirmation of cutaneous manifestation due to Brucella melitensis. J Med Microbiol 56: 283-285 [Abstract] [Full Text]  
  • Wellinghausen, N., Nockler, K., Sigge, A., Bartel, M., Essig, A., Poppert, S. (2006). Rapid Detection of Brucella spp. in Blood Cultures by Fluorescence In Situ Hybridization.. J. Clin. Microbiol. 44: 1828-1830 [Abstract] [Full Text]  
  • Pappas, G., Akritidis, N., Bosilkovski, M., Tsianos, E. (2005). Brucellosis. NEJM 352: 2325-2336 [Full Text]  
  • Gee, J. E., De, B. K., Levett, P. N., Whitney, A. M., Novak, R. T., Popovic, T. (2004). Use of 16S rRNA Gene Sequencing for Rapid Confirmatory Identification of Brucella Isolates. J. Clin. Microbiol. 42: 3649-3654 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»