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Journal of Clinical Microbiology, March 2004, p. 1298-1301, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1298-1301.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Hepatitis C Virus Isolates from Argentina Disclose a Novel Genotype 1-Associated Restriction Pattern

María Inés Gismondi,1* Lothar Heinrich Staendner,2 Saúl Grinstein,1 Carlos Alberto Guzmán,2 and María Victoria Preciado1

Laboratorio de Virología, Hospital de Niños Ricardo Gutiérrez, C1425EFD Ciudad de Buenos Aires, Argentina,1 Vaccine Research Group, Division of Microbiology, Gesellschaft für Biotechnologische Forschung- German Research Centre for Biotechnology, D-38124 Braunschweig, Germany2

Received 4 September 2003/ Returned for modification 9 November 2003/ Accepted 11 November 2003

Hepatitis C virus isolates which disclosed a novel genotype 1-associated restriction pattern by restriction fragment length polymorphism analysis were characterized. Except for a mother and child pair, the patients were unrelated. Sequence analysis showed a G->A substitution leading to a new RsaI recognition site. Phylogenetic analysis revealed that these isolates constitute a novel genetic lineage within the main cluster of genotype 1 strains.

* Corresponding author. Mailing address: Laboratorio de Virología, Hospital de Niños Ricardo Gutiérrez, Gallo 1330, C1425EFD Ciudad de Buenos Aires, Argentina. Phone: 5411-4964-3118. Fax: 5411-4962-6770. E-mail: migismondi{at}yahoo.com.ar.

Journal of Clinical Microbiology, March 2004, p. 1298-1301, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1298-1301.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2004 by the American Society for Microbiology. All rights reserved.