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Journal of Clinical Microbiology, March 2004, p. 1302-1304, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1302-1304.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Stability of Polymorphic GC-Rich Repeat Sequence-Containing Regions of Mycobacterium tuberculosis

Madalene Richardson,1* Gian D. van der Spuy,1 Samantha L. Sampson,1,{dagger} Nulda Beyers,2 Paul D. van Helden,1 and Robin M. Warren1

MRC Centre for Molecular and Cellular Biology, Department of Medical Biochemistry,1 Centre for TB Research and Education, Department of Paediatrics and Child Health, Faculty of Health Sciences, Stellenbosch University, Tygerberg, South Africa2

Received 28 April 2003/ Returned for modification 28 July 2003/ Accepted 17 November 2003

Mycobacterium tuberculosis cultures were subjected to DNA fingerprinting with IS6110- and polymorphic GC-rich sequence (PGRS)-containing probes. The PGRS banding patterns remained highly stable during multiple cultures of specimens from one disease episode (0.5% changed) and during transmission in patients with close contact (1.9% changed). Characteristic PGRS-restriction fragment length polymorphism motifs for different strain groupings may indicate distant evolutionary events leading to the differentiation of M. tuberculosis strain lineages.

* Corresponding author. Mailing address: P.O. Box 19063, Tygerberg 7505, South Africa. Phone: 27 21 9389073. Fax: 27 21 9389476. E-mail: mr6{at}sun.ac.za.

{dagger} Present address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Mass.

Journal of Clinical Microbiology, March 2004, p. 1302-1304, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1302-1304.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Machowski, E. E., Barichievy, S., Springer, B., Durbach, S. I., Mizrahi, V. (2007). In Vitro Analysis of Rates and Spectra of Mutations in a Polymorphic Region of the Rv0746 PE_PGRS Gene of Mycobacterium tuberculosis. J. Bacteriol. 189: 2190-2195 [Abstract] [Full Text]  


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