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Journal of Clinical Microbiology, March 2004, p. 1326-1329, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1326-1329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Direct Identification of Group B Streptococcal Alpha-Protein-Like Protein Genes

Roberta Creti,* Francesca Fabretti, Graziella Orefici, and Christina von Hunolstein

Laboratorio di Batteriologia, Istituto Superiore di Sanità, 00161 Rome, Italy

Received 3 October 2003/ Returned for modification 10 November 2003/ Accepted 4 December 2003

We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.

* Corresponding author. Mailing address: Laboratorio di Batteriologia, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49902036. Fax: 39 06 49387112. E-mail: roberta.creti{at}iss.it.

Journal of Clinical Microbiology, March 2004, p. 1326-1329, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1326-1329.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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