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Journal of Clinical Microbiology, March 2004, p. 1337-1340, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1337-1340.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Klebsiella pneumoniae from Blood Culture Bottles by Real-Time PCR

Prathiba Kurupati,1 Carol Chow,2 Gamini Kumarasinghe,2 and Chit Laa Poh1*

Department of Microbiology, National University of Singapore, Singapore 117597,1 Department of Laboratory Medicine, National University Hospital, Singapore 119074, Republic of Singapore2

Received 14 July 2003/ Returned for modification 27 August 2003/ Accepted 17 November 2003

A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to results of conventional biochemical characterization. The assay also showed 100% sensitivity, as it correctly identified all 142 positive-control blood cultures and 4 from double-blind trials.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Republic of Singapore. Phone: (65) 687-43674. Fax: (65) 677-66872. E-mail: micpohcl{at}nus.edu.sg.

Journal of Clinical Microbiology, March 2004, p. 1337-1340, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1337-1340.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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