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Journal of Clinical Microbiology, March 2004, p. 1353-1355, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1353-1355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Ehrlichial DNA in Haemaphysalis Ticks Recovered from Dogs in Japan That Is Closely Related to a Novel Ehrlichia sp. Found in Cattle Ticks from Tibet, Thailand, and Africa

Hisashi Inokuma,1* Takeshi Beppu,1 Masaru Okuda,1 Yojiro Shimada,2 and Yoshimi Sakata3

Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515,1 Nippon Zenyaku Kogyo Co., Ltd., Koriyama, Fukushima 963-0196,2 Merial Japan, Ltd., Tokyo 100-0014, Japan3

Received 30 June 2003/ Accepted 12 November 2003

Tick DNA samples from dogs in Japan were examined for Ehrlichia infection by 16S rRNA gene-based PCR and sequencing. Three positive samples were detected from Haemaphysalis ticks, and higher levels of similarity (98.46 to 99.06%) were found to recently detected Ehrlichia spp. from cattle ticks in Tibet, Thailand, and Africa.


* Corresponding author. Mailing address: Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515 Japan. Phone and fax: 81-83-933-5895. E-mail: inokuma{at}yamaguchi-u.ac.jp.


Journal of Clinical Microbiology, March 2004, p. 1353-1355, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1353-1355.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.







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