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Journal of Clinical Microbiology, March 2004, p. 1360-1362, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1360-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of a Diagnostic PCR Assay Targeting the Mn-Dependent Superoxide Dismutase Gene (sodA) for Identification of Streptococcus gallolyticus

Eiki Sasaki,1 Ro Osawa,1* Yosuke Nishitani,1 and Robert A. Whiley2

Department of Bioscience, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501, Japan,1 Department of Clinical and Diagnostic Oral Sciences, Barts and The London, Queen Mary's School of Medicine and Dentistry, London E1 2AD, United Kingdom2

Received 12 August 2003/ Returned for modification 10 November 2003/ Accepted 3 December 2003

A PCR-based assay to identify Streptococcus gallolyticus has been developed. The assay uses an oligonucleotide primer pair targeting a partial sequence of the manganese-dependent superoxide dismutase gene (sodA). The assay distinguished members of the S. gallolyticus group from other, closely related taxa successfully by yielding a 408-bp specific amplicon.

* Corresponding author. Mailing address: Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501. Phone and fax: 81-78-803-5804. E-mail: osawa{at}ans.kobe-u.ac.jp.

Journal of Clinical Microbiology, March 2004, p. 1360-1362, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1360-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Onoyama, S., Ogata, R., Wada, A., Saito, M., Okada, K., Harada, T. (2009). Neonatal bacterial meningitis caused by Streptococcus gallolyticus subsp. pasteurianus. J Med Microbiol 58: 1252-1254 [Abstract] [Full Text]  
  • Chen, H.-J., Tsai, J.-C., Chang, T.-C., Hung, W.-C., Tseng, S.-P., Hsueh, P.-R., Teng, L.-J. (2008). PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences. J Med Microbiol 57: 432-438 [Abstract] [Full Text]  
  • Osawa, R., Sasaki, E. (2004). Novel Observations of Genotypic and Metabolic Characteristics of Three Subspecies of Streptococcus gallolyticus. J. Clin. Microbiol. 42: 4912-4913 [Full Text]  


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